Medical Interventions

Bellringers Second Quarter

If you begin with a 100 mg/dl solution of glucose in tube 1 and make four 1;2 dilutions, what is the concentration of glucose in each tube?

fc: 1.) 1,000 mg/dl

2.) 500 mg/dl

3.) 250 mg/dl

4.) 125 mg/dl

Show the tube and final dilutions of a 6 ng/ml sample, that is diluted by taking 10 μl into a 40 μl four times. also show the concentration of each tube.

td: 1.) 1/5

2.) 1/5

3.) 1/5

4.) 1/5

fd: 1.) 1/5

2.) 1/25

3.) 1/125

4.)1/625

fc: 1.) 6 ng/ml

2.) 1.2 ng/ml

3.) 0.24 ng/ml

4.) 0.048 ng/ml

Why is it important for doctors to know the concentration of a disease antigen present in a patient’s system?

Knowing the concentration helps point to who contracted the disease first, how the disease was spread between patients, and how strong the disease is.

When using a micropipette to transfer liquid from the original tube to a new tube if you push to the second stop, what will occur? Why is this step important?

Pushing to the second stop dispenses the exact volume of liquid needed from the micropipette. This step is crucial for accurate measurements and ensures consistency in experiments.

Summarize the results of the test. Include an estimate of the amount of antigen in the patient samples

Patient 1: Positive, had concentration of 32-64 ng/ml

Patient 2: negative, had concentration of 0-2 ng/ml

Disease samples from two patients were collected and subjected to serial dilutions before running an ELISA. What does it mean if the disease can be detected in samples from one person only at a dilution of 1/5, but the disease can be detected in the other patient at a dilution of 1/5 and 1/100.

This shows that the first patient has a higher concentration of the disease antigen in their sample compared to the second patient.

In sudden outbreaks, does the ELISA detect antigens or antibodies? Why?

The ELISA detects antigens so that we can see who has a disease and how high of a concentration they may have of it

What is the purpose of the control wells in the ELISA test?

To visually display how high the concentration of the antigen is in the samples being tested compared to known standards.

Explain the job of a chief data science officer and why it is important.

What degree is needed and what is the salary?

Professionals who organize large quantities of data in different ways (graphs, plots, and visualizations). This job is important because they find information that pertains to financial, health, and geospacial issues.

Bachelor’s in computer science, data science, and makes annual salary of $161,020