DNA Cloning and Characterization

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Last updated 11:39 PM on 9/9/25
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4 Terms

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What is recombinant DNA technology?

A method where plasmids and restriction enzymes are used to generate DNA molecules carrying inserted genes or DNA fragments that replicate in host cells.

2
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What are DNA libraries?

Collections of recombinant DNA molecules representing all genes (genomic library) or expressed mRNAs (cDNA library) of a cell type.

3
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What is PCR used for?

To amplify specific DNA sequences and assist in DNA sequencing.

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How are whole genome sequences assembled?

By sequencing a large number of overlapping DNA clones and piecing them together.