Photosynthesis
process to create glucose from carbon dioxide and water, using light energy from the sun
Glucose
C6H12O6
Insoluble starch
complex carbohydrate made from products of photosynthesis and used for storage
Chloroplast
organelle where photosynthesis occurs
Chlorophyll
green pigment that absorbs light energy used to carry out photosynthesis
Light intensity effect
Photosynthesis rate increases as more energy is available, up to a certain point
Carbon dioxide concentration effect
Photosynthesis rate increases as more carbon is available, up to a certain point
Photosynthetic rate
speed which plants take in reactants to produce products
Testing leaves for starch
Plant leaves can be tested for the presence of starch using iodine solution, after the leaf has been intensely heated and submerged in ethanol
Iodine test for starch
change colour from orange to blue-black
Starch results for plants kept in light conditions
Plants store glucose as starch in the leaves, plants that are kept in the light will photosynthesise and store starch so their leaves will change colour to blue-black
Starch results for plants kept in the dark
Plants in the dark will be unable to photosynthesise meaning glucose will not be made and stored as starch, so iodine will not change colour
Starch results for variegated leaves
Leaves that are variegated have patches absent of chlorophyll, these regions will not cause iodine to change colour as photosynthesis cannot occur here
Investigating photosynthesis in pondweed
Aquatic plants produce observable bubbles of oxygen when photosynthesising near light
Pondweed bubbles
presence of oxygen from photosynthesis, more bubbles =greater rate
Light intensity and rate of photosynthesis in pondweed
closer=greater light intensity=increase the rate of photosynthesis
Sodium hydroxide
A basic solution that can break down carbon dioixde
Effect of plant kept enclosed near sodium hydroxide
a sealed bag with sodium hydroxide will be unable to photosynthesise due to a lack of carbon dioxide
Photosynthesis
A process where plants and algae synthesise glucose from carbon dioxide and water, using light energy from the sun
Photosynthesis word equation
Carbon dioxide + water (light energy+chlorophyll) -> glucose + oxygen
Balanced equation for photosynthesis
6CO2 + 6H2O -> C6H12O6 + 6O2
Oxygen
6O2
Carbon dioxide
6CO2
Water
6H2O
Endothermic reaction
energy is taken in from surrounding environment
Temperature effect
Photosynthesis rate increases to an optimum and then decreases due to denaturing enzymes
Light intensity effect
Photosynthesis rate increases as more energy is available, up to a certain point
Carbon dioxide concentration effect
rate increases as more is available up to a certain point
Cellulose
Carbohydrate component of plant cell walls made from products of photosynthesis
Amino acids
Building blocks of protein made from products of photosynthesis
Enzymes
should be maintained in optimal conditions for efficient rates of photosynthesis
Adding a leaf to boiling water during a starch test
Kills the leaf to prevent any further reactions that might break down starch or affect its presence
Adding hot ethanol during a starch test
remove chlorophyll from the leaf
Washing the leaf before examination during a starch test
Softens the leaf
Risk of starch tests
using bunsen burners, ethanol is very flammable and should be kept away from flames
Dependent variable
The variable that is measured, in this case the presence of starch or colour of iodine test
Independent variable
The variable that is changed such as the type or source of leaf
Control variables
The variables that are kept the same
Temperature
Increases the rate of reaction up to an optimum rate, after which the denaturation of enzymes will occur
Sunlight
Is needed as an energy source for photosynthesis to occur
Carbon dioxide
Enters the plant through stomata is the source of carbon needed to make glucose
Temperature effect
Photosynthesis rate increases to an optimum and then decreases due to denaturing enzymes
Light intensity effect
Photosynthesis rate increases and then plateaus due to another limiting factor such as temperature or carbon dioxide
Carbon dioxide concentration effect
Photosynthesis rate increases and then plateaus due to another limiting factor such as temperature or light intensity
Limiting factor
An environmental factor that prevents photosynthetic rate from increasing
Three main limiting factors
Temperature, light intensity and carbon dioxide concentration
Plateau
Where a graph levels off and no longer increases past a certain point
Inverse-square law
Light intensity is inversely proportional to the square of the distance from a light source
Inversely proportional relationship
As the distance of light from a plant increases, the light intensity decreases
Purpose of photosynthesis
products used for respiration and to make new molecules
Purpose of respiration
to obtain energy, used for growth, new molecules, movement in animals, and temperature regulation
Aerobic respiration
take in glucose from food and oxygen from the air to release energy, products released into the air
When photosynthesis occurs
In light conditions during the day, assuming conditions are available
When respiration occurs
All the time in animals and plants, plant respiration is more observable at night as they do not photosynthesise at this time
Gas exchange
swapping of gases, can occur in the stomata or the lungs
Hydrogencarbonate indicator
detect increases and decreases of carbon dioxide concentration, to interpret the effect of gas exchange
Hydrogencarbonate indicator colour changes
normally red, will change to purple in low concentrations and yellow in high
In light conditions
rate of photosynthesis is higher than respiration, net gas exchange oxygen out
Hydrogencarbonate indicator result for light conditions
purple
At dusk or dawn
light intensity is low the rates of photosynthesis and respiration are equal, known as the compensation point with no net gas exchange, CO2 0.03%
Hydrogencarbonate indicator result for low light conditions (dusk/dawn)
will be red because no carbon dioxide change will be detected, compensation point has been achieved where respiration occurs at the same rate as photosynthesis
In dark conditions
Only respiration is occurring as there is no light for photosynthesis
Hydrogencarbonate indicator result for dark conditions
turns to yellow as rate of photosynthesis is lower than the rate of respiration
Mesophytic leaf
adapted to general conditions, those that are not too dry or too humid
Epidermal tissue
covers and protects the plant, physical defence, transparent to allow light through
Waxy cuticle
protect plant and reduce water loss, thicker on upper surface, transparent
Palisade mesophyll
below epidermis, compacted together and contain high quantities of chloroplasts
Spongy mesophyll
Layer of tissue beneath palisade mesophyll, packed loosely for efficient gas exchange with increased surface areas
Intercellular space
space between to provide room for increased rate of diffusion
Stomata
Small openings on the underside of a leaf
Role of the stomata
opens to allow gas exchange
Guard cells
Pairs of cells that surround the stomata and control their opening and closing to optimise gas exchange and reduce water loss
Activity of guard cells
open stomata in light conditions and close at night
Xylem
Non-living vascular tissue that carries water and dissolved minerals from the roots of a plant to its leaves, the xylem is hollow and nearby cells are lignified or dead so water can move continuously
Phloem
Living and elongated vascular tissue that carries sugar and organic substances throughout a plant, the phloem has sieve plates that act as pores to allow molecules to diffuse between cells