Pneumonia

Penecilin is a chemical mimic of?

D-ala, D-ala

What does penicillin inhibit?

transpeptidase activity

What sort of inhibit is penicillin

Irreversible competitive

Cell wall under?

Lots of tension/pressure

Organisation of cell wall factors?

Clusters

Classic genetics uses?

Random mutagenesis

Dr Leukenhaus did?

Forward genetics

What phenotype to study essential processes?

Conditional

complementation uses?

Lambda phage with random chunks of WT e.coli genome

Random mutagenesis

Bigger the gene, more likely to get a mutation in it

FtsZ

Tubulin homologue

Issue with FtsZ therapeutics?

Not excreted fast enough. Puts pressure on kidneys

Impact of deleting MreB

Cells die by osmotic lysis

Used what to study MreB

Xylose inducible promoter

A22 effect on MreB

MreB becomes diffuse. Swelling and lysis of the cell

MreB structure

Monomers form patches that travel around the circumference of the cell

Tagged MreB with?

Anti cMyc

How were PBP regulatory factors discovered?

Synthetically lethal genetics

PBP role?

Last steps of cell division and elongation complex

What do both pbp1a and b use?

Lipid II linked precursor

Modern Micro needs?

Strip back redundancy

KO pbp1b in e.coli?

Pbp1a becomes essential

What did synthetic lethal pairs in e.coli use?

Unstable plasmids and transposon mutagenesis

What did the e.coli plasmid contain?

Pbp1b and lacZYA

Transponson jumped into lpoA

Pbp1b on plasmid becomes essential

Transposon jumped into a gene synthetically lethal with pbp1b

Whole plasmid becomes essential. Get a solid blue colony

Synthetic lethal pair with pbp1b

LpoA

Synthetic lethal pair pbp1a

LpoB