bio lab quiz 3 - BIO 1112 Northeastern University

order of substance during the Gram staining procedure

1. Crystal Violet

2. Gram Iodine

3. Ethanol/Acetone

4. Safranin

gram positive bacteria

show blue or purple after gram-staining in a laboratory test and have thick cell walls

gram negative bacteria

show pink or red on staining and have thin walls

what is the bacterial cell wall made of

peptoglycan

what part of gram staining is actually stained

cell wall

Spirillum

spiral

cocci

spherical

bacillus

rods

purpose and procedure of quadrant streaking technique and the preparation of a bacterial lawn

technique used to isolate specific colonies - preparation includes covering the entire plate with a bacterial lawn

how do prokaryotes get energy

light, inorganic chemicals, nutrutrients from environment

how do prokaryotes reproduce

binary fusion - cells duplicate their components and divide - become indepdents yet attached

what does crystal violet do

Color Cells Purple

what does gram iodine do

Sets the color of the stain

what does Ethanol/Acetone do

Decolorizes; removes stain from Gram Negative bacteria

what does safranin do

Counterstain Gram Negative cells