Section 3: DNA Replication Mechanics

Part 3

Which protein initiates unwinding at oriC in E. coli?
A) DnaA
B) DnaB
C) DnaC
D) Gyrase

A

DnaB functions as:
A) Initiator protein
B) Helicase
C) Topoisomerase
D) Primer synthase

B

The enzyme that relieves supercoiling tension is:
A) Topoisomerase (DNA gyrase)
B) Helicase
C) Ligase
D) Polymerase

A

Which proteins prevent reannealing of DNA during replication?
A) DnaA
B) SSBPs
C) Primase
D) Ligase

B

The enzyme Primase synthesizes:
A) DNA primer
B) Okazaki fragments
C) Protein initiation complex
D) RNA primer

D

The leading strand is synthesized:
A) Discontinuously away from the fork
B) Using Okazaki fragments
C) Bidirectionally
D) Continuously toward the fork

D

The lagging strand is synthesized:
A) Continuously toward the fork
B) From RNA template
C) Without primers
D) Discontinuously away from the fork

D

Okazaki fragments are joined together by:
A) DNA ligase
B) Primase
C) DNA polymerase III
D) Gyrase

A

Which process corrects mismatched bases during replication?
A) Proofreading
B) Supercoiling
C) Priming
D) Ligation

A

The proofreading direction of DNA polymerase is:
A) 5’ → 3’
B) 3’ → 5’
C) Bidirectional
D) 5’ → 5’

B