toxin that inhibits SERCA resulting in reduced ER lumen Ca++
thapsigargin
toxin that inhibits the attachment of the first N-acetylglucosamine during the synthesis of the assembly of the oligosaccharide for N-linked glycosylation
tunicamycin
primary organizing protein associated with all protein-folding activities within the rER lumen
Bip
maintains the monomeric state of IRE and PERK
Bip
exerts a kinase action on eIF2alpha
PERK
expressed protein of the unfolded protein response (aka ER-stress response) most closely associated with linking it to apoptosis
CHOP
anti-apoptotic protein lowered in expression in response to CHOP action
Bcl-2
pro-apoptotic protein raised in expression by p53
Bax
leaks out of the mitochondrion through Bax-ringed "outer membrane permeability pores" with Apaf-1 to form the apoptosome
cytochrome c
dimerized by the apoptosome to cross-cleave and produce two active enzymes
procasapase-9
cuts at the sequence ...DAVD... within ICAD
caspase-3
enzyme directly producing multiple DNA fragments in terminal stages of apoptosis
CAD
stratum _______ layer of the epidermis whose characteristic appearance (giving rise to its name) results from lowered expression of Bcl-2
granulosum
reactive amino acid within caspase active sites
cysteine
partner amino acid within caspase active sites that withdraws a proton from the reactive amino acid
histidine
used to stabilize the protonated partner during the reaction cycle of caspase-9
glutamate
reporter enzyme used to judge the degree of CHOP regulated expression from a Bcl2-promoter
chloramphenicol acetyl-transferase
assay that most directly monitors the level of transcription of the Bcl2 gene
RT-PCR
used to separate 14-C labeled chloramphenicol from acetylated forms
thin-layer chromatography
live cell membrane-impermeable stain that is not excluded by apoptosed cells
propidium iodide
transgenically expressed clotting factor in CHO cells that induces the unfolded protein response and is poorly secreted
wtFVIII
highly reduced size of the transgenically expressed clotting factor missing the entire fragment which has a large number of targets for N-linked glycosylation
BDD
reduced size of the transgenically expressed clotting factor with only 6 closely spaced targets for N-linked glycosylation within the molecularly modified B domain
226/N6
form of transgenically expressed clotting factor that is best secreted by CHO cells
226/N6
ER lumen protein which bonds to N-linked glycosylations in which terminal glucoses have been retained
calnexin
inhibitor of NOSs which results in higher production of superoxide and less NO
ADMA
modifier of C674 of SERCA that enables glutathiolation
peroxynitrite
modification of SERCA C674 that prevents glutathiolation in atherosclerotic tissue
sulfonation
form of SERCA C674 that is reactive with iodoacetamide
sulfhydryl
modification of C674 that increases SERCA action
gluthiolation
substrate used to identify fragmented DNA with reactive 3'-OHs during TUNEL assays
dUTP-5-fluorescein
enzyme used to label fragmented DNAs in TUNEL assays
terminal deoxynucleotidyl transferase
enzyme that produces reactive 3'-OH's in apoptosis
CAD
virus used to create transformed CHO cell lines and integrate exogenous genes (such as FVIII) for expression
SV40
substance that is fluorescently non-responsive but can be oxidized within the cell by reactive oxygen species to become fluorescently green
dichlorofluorescin-H2
antioxidant found in many food products (notably breakfast cereals) that reduces the unfolded protein response to FVIII overexpression
butylated hydroxyanisole
substance that activates expression from the SV40 late promoter allowing production of transgenic gene products (such as FVIII)
sodium butyrate
used to count the numbers of cells that display colors that are instrumentally detected usually associated with some variable phenomenon
FACS
control applied substance used to oxidize dichlorofluorescein in CHO cells
hydrogen peroxide
protein expressed experimentally in CHO cells that results in the highest DCF fluorescence in CHO cells
wtFVIII
substance used to lower DCF fluorescence in CHO cells caused by overloading the ER
butylated hydroxyanisole
enzyme that reduces hydrogen peroxide using GSH
glutathione peroxidase
oxidized product of glutathione peroxidase
GSSG
breakdown product produced in cells in which peroxide has reacted with lipids
malondialdehyde
SV40 late promoter driven expression of _______ produces the highest level of ROS
wtFVIII
SV40 late promoter driven expression of which form of a clotting factor produces the lowest level of ROS
226/N6
podocytes (specialized endothelial cells of the glomerulus that sieve out cells and large macromolecules allowign only fluid flow into nephrons) produces what product that reacts with DAF-FM Diacetate to cause it to fluoresce green
NO
ADMA-treated glomeruli take up what provided macromolecule (experimentally) unlike untreated glomeruli
bovine serum albumin
ADMA-treated glomeruli that have taken up provided macromolecule from solution quantifiably swell when now dipped in ____
water
the ____ of a swollen ADMA-treated glomerulus is a measure of its damage
diameter
ADMA damage to glomeruli can be reduced and reversed in response to NO produced by varying concentrations of:
deta-NONOate
NO stimulates what enzyme most directly
guanylate cyclase
what activator of PKG can cross the cell membrane and reduce the degree of ADMA damage to glomeruli
Br-cGMP
what compound oxidizes the heme of soluble guanylate cyclase
ODQ
TEMPOL is a scavenger for _____ which prevents ADMA damage to glomeruli
superoxide