immobilising enzymes

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5 Terms

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enzyme immobilisation

the attachment of enzymes to insoluble materials, which then provide support for the enzymes

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how support is provided

holds enzymes in place during reactions…allow for easy separation and reuse

3
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lactase immobiliazation

trapped in alginate beads

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process of immobilization

mix enzyme with sodium alginate…drip mixture through syringe into calcium chloride solution…sodium ions displaced by calcium ions forming insoluble beads of calcium alginate…harden beads and rinse

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advantages of immobilization

no product and enzyme contamination…longer enzyme shelf life…more stable enzyme…more productive enzyme…easier downstream processing…les time consuming process