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The following are factors considered to warrant tests EXCEPT:
Body site
Presence of other bacte
Spn quality
Host status
NOTA
NOTA
T/F
Susceptibility test can be performed even if normal flora is present
False
T/F
The antibiotic needs to access the body site
True
The following are a must in performing susceptibility test EXCEPT
Pure culture
No contaminant
Both
Neither
Neither
The presence numerous colony morphotypes may indicate _____
Heavy contamination
What is used for susceptibility test on immunocompromised hosts
Normal flora
Selecting antimicrobial agents for tests should involve what 3 departments
Lab personnel
Infectious disease
Pharmacy
Susceptibility test method prepared from a single/several isolated colonies of similar morphology
Inoculum preparation
Highly fastidious spn are suspended in the following EXCEPT:
Water
NSS
Broth medium
NOTA
Broth medium
Recommended & most common method for highly fastidious spn
Direct suspension
Which of the following is false:
Inoculum is compared to control
Turbidity of inoculum must match standard
Inoculum must be used within 15 mins
NOTA
Inoculum is compared to control
Inoculum is compared to standard
Standard prepared by adding 1% sulfuric acid & 1.175% barium chloride
McFarland Turbidity solutions
What is used for turbidity standards to provide longer shelf life
Latex particles
Standard comparable to bacterial suspension of 1.5×10^8 CFU/ml
McFarland 0.5 standard
Newer preparations use latex particles instead of _____
Barium sulphate precipitates
What is determined in dilution method
Minimal inhibitory conc (MIC)
Refers to lowest conc of agent required to inhibit growth
Minimum inhibitory conc (MIC)
Broth macrodilution is also known as
Tube dilution
Dilution method using 2 fold serial dilution
Broth macrodilution
Broth macrodilution must attain final conc of
5×10^5 CFU/ml
The gold standard dilution method
Broth dilution
What does broth microdilution use in place of test tubes
Microtiter plates
2 difficulties of broth dilution
Trailing growth
Skipped wells
Refers to 80% reduction in MIC
Trailing growth
Error in preparation, small resistant population, & contamination can cause ____
Skipped wells
Series of plates containing various conc of antimicrobial inoculated with test bacte
Agar dilution test
An example of multi pronged replicating device
Steer’s replicator
Swabbing standardized inoculum & applying disks containing single conc of antibiotics
Disk diffusion/kirby bauer
Agar used in kirby bauer test
Mueller Hinton agar (4mm deep)
When are zones of inhibition read
After overnight incubation
Most common & versatile non automated dilution method
Kirby bauer
Are resistant to erythromycin & susceptible to clindamycin
S. aureus
Erythromycin & clindamycin are placed ____ mm apart
15-26
Indicates inducible clindamycin resistance & isolate is reported clindamycin resistant
D zone of inhibition
Note 
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