Protein Purification I

These flashcards cover key concepts and processes related to protein purification methods, including terminology and specific techniques discussed in the lecture.

Protein purification generally refers to strategies used to separate one protein from a __ of many other proteins.

mixture

The sources of protein for purification can include __, tissue culture cells, yeast, bacteria, and plants.

mammalian organs

If your protein of interest is ___, you need to break the cells open.

cytosolic

The first step in the purification of most proteins is to disrupt __ and cells in a controlled fashion.

tissues

Homogenization is a mechanical procedure that ruptures plasma __ of cells.

membranes

In differential ___, cells are separated into their components by centrifugation at increasing speeds.

centrifugation

Centrifugal force is required to separate smaller and less dense components into the __ and pellet.

supernatant

Salting out refers to the differential __ of proteins at high salt concentrations.

solubility

Dialysis can be used to remove __ molecules from a solution.

small

In gel filtration chromatography, proteins are separated based on __, with large proteins eluting first.

size

Column chromatography utilizes packed matrices to separate proteins based on their different __.

properties

Gel filtration chromatography is also known as __ exclusion chromatography.

size

The technique of adding ammonium sulfate to precipitate proteins is called __ out.

salting

Alcohol dehydrogenase (ADH1) in yeast converts acetaldehyde to __ during fermentation.

ethanol

When performing purification, you should keep all samples on __ at all times to prevent degradation.

ice

In the purification protocol, the specific activity of a protein is expressed in __ per milligram of protein.

units