Semen

Site of spermatogenesis

Seminiferous tubules

Site of sperm maturation

Epididymis

Nurse cells for developing sperms

Sertoli cells

Earliest stage of sperm maturation

Spermatogonium

Majority of seminal fluid comes from _

Seminal vesicles

Identify part of testes:

• provides nutrients

• rich in fructose

Seminal vesicles

Identify part of testes:

• acidic, contains ACP

• for coagulation and liquefaction

Prostate fluid

Identify part of testes:

• neutralizes acidity

Bulbourethral glands

Cowper’s glands are AKA

Bulbourethreal glands

Abstinence for semen collection

2-7 days

Specimen for semen analysis must be delivered to lab within _

1 hr

Specimens awaiting analysis should be kept at what temo\p

37C

The gray-white transparent of semen is due to _

Flavin

Normal volume of semen

2-5mL

0 Watery; 4 gel-like reports what?

Viscosity

Normal pH of semen

7.2-8.0

Normal value of sperm conc.

>20 million sperms/mL

Cold tap water is used as a reagent for what

Diluent for Sperm conc.

This is a counting chamber for sperm concentration for undiluted specimen and uses heat to immobilize sperm

Makler counting champer

Shortcut method for sperm conc. computation

2 WBC squares: # sperms counted x 100,000

5 RBC squares = # sperms counted x 1,000,000

Normal sperm count

>=40 million per ejaculate

Formula for sperm count

Sperm conc. x Specimen volume

Identify the sperm motility grading:

• Rapid-straight line motility

4.0

Identify the sperm motility grading:

• SlowER speed, some lateral movement

3.0

Identify the sperm motility grading:

• Slow forward progression, noticeable lateral movement

2.0

Reagent of Modified Bloom’s test (Sperm viability)

Eosin and Nigrosin

Decreased Neutral a-glucosidase is associated with what disorder

Epididymis disorder

MAR and Immunobead tests for what

Antisperm antibodies

MAR detects what Ig

IgG

Immunobead test detects what Ig

IgGAM

Test for spermine (very specific)

Barbiero’s test

Test for choline (not specific)

Florence test

Post-vasectomy semen analysis done after how many months

2 months

2 hormones that stimulate spermatogenesis

FSH and LH

Grade the Sperm Motility:

• Rapid, straight line motility

4.0

a

Grade the Sperm Motility:

• Slower speed, some lateral movement

3.0

b

Grade the Sperm Motility:

• Slow forward progression, noticeable lateral movement

2.0

b

Grade the Sperm Motility:

• No forward progression

1.0

c

Grade the sperm motility (in alternative format):

• Sperm moving linearly or in a large circcle

Progressive

Grade the sperm motility (in alternative format):

• Sperm moving with an absence of progression

Nonprogressive

Stain/test for sperm viability/vitality

Eosin-nigrosin stain

Test to do for sperm if there is lack of seminal vesicle support medium

Fructose level

Mixed agglutination reaction and immunobead tests are to test for what abnormality

Male antisperm antibodies