- question/problem - what gets you started - must be reproducible - observation + description, measurement, or record of any object/phenomenon
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step 2 of scientific method: hypothesis + requirements
- bold statement --\> NOT a question (educated guess) - 2 requirements: testable and falsifiable/refutable - informed, logical, and plausible explanation for observations of the natural world - can NEVER be proven, only support it
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step 3 of scientific method: prediction
- tells what to expect if hypothesis is supported - "if...then..." statement - if \= independent variable - then \= dependent variable - doesn't mean you're gonna get it, just a prediction of what will happen
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step 4 of scientific method: experiment
- repeatable manipulation of one or more aspects of the natural world - must be very detailed, comprehensive, consistent, and reduce bias
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variable
anything that can change (in an experiment)
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independent variable
that which the scientist manipulates (I change)
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dependent variable
any variable that responds or could potentially respond to changes in the independent variable (what's being measured)
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control
baseline for comparison that doesn't change during the experiment and doesn't affect the dependent variable
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constants
factors that stay the same throughout the entire experiment (ex: amount of food, water, etc.)
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correlation
two or more aspects of the natural world behave in an interrelated manner
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is this example a causation or correlation? ex: the precent of high schoolers graduating and moving onto college and an increase in global average temperatures
correlation
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causation
one variable causes the change in the second variable
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is this example a causation or correlation? ex: as population increases the more high school graduate move onto college
causation
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what is a scientific claim?
- statement that's made about the natural world that can be tested using the scientific method - anyone can make one - many of them aren't true
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what is scientific literacy and why is it important?
- it's an understanding of the basics of science and the scientific process - it's important because it enables us to make informed decisions about the world around us; it also enables us to communicate our knowledge to others
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4 major things to consider when evaluating scientific claims
1. real vs pseudo science 2. bias/agenda 3. peer review/publication 4. credentials
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real vs pseudo science
- real: follows scientific method - pseudo: characterized by scientific-sounding statements, beliefs, or practices that aren't based on the scientific method
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bias/agenda
- bias: a prejudice or opinion for or against something - this is bad/very dangerous
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applied research
- businesses and industries provide the majority of funding to find practical uses for scientific discoveries - money is a strong factor/motivator to support or reject a claim
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basic research
- intended to expand fundamental knowledge of science - largely funded by federal government
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types of literature
1. primary 2. secondary 3. social media
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primary literature
- first publication of scientific research that contains actual data with controls (includes peer review) - ex: technical reports, conference proceedings, dissertations, peer-reviewed science journals
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secondary literature
- summary of primary literature; sources that give a basic overview of topic or summarizes research results (must cite original study and have credible credentials) - ex: our textbook, review articles, popular scientific journals, respected institutions on the internet (like National Geographic)
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social media
- literally anything else - NOT a good source - ex: twitter, instagram reels, TikTok, Facebook posts, etc.
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everything is made up of \__________ (anything that has mass and takes up space)
matter
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each piece of unique matter we can an \_________ - a pure substance that has distinct properties and cannot be broken down
element
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the smallest amount of an element (in pure form) is called an
atom
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together atoms make
molecules
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what element is found in living organisms that is rare in non-living things?
carbon
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isotope practice problem: nitrogen-11 is an isotope of nitrogen that has 7 protons and 4 neutrons. what is the atomic number and atomic mass number (atomic weight) of nitrogen-11?
- atomic number: 7 - atomic mass number: 11
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what is an isotope?
forms of atoms that have the same number of protons, but differing number of neutrons; difference \# of neutrons means differing weight and unstable nucleus
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covalent bonding
sharing one or more electrons to form molecules
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ionic bonding
giving or receiving electrons
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hydrogen bonding
- WEAK covalent bond - uneven distribution of charge that allows water to form weak bonds - almost every chemical reaction that occurs in living organisms is associated with water - water has special properties such as being a polar molecule
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hydrophobic
- water-fearing - substance doesn't dissolve in water - non polar molecules
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hydrophilic
- water loving - substance dissolves in water - polar molecules
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biomolecules
- big molecules that pertain to life - contain at least carbon-hydrogen bond
- composed of monosaccharides (single sugars) held together by covalent bonds - function: short-term energy storage and immediate-term energy
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proteins
- polypeptide chains that are folded into 3D shapes - composed of amino acids that are held together by peptide bonds - order of amino acids matter because it determines the protein that's made and its specific function - if protein loses its shape, it loses its function \= this is when it's denatured - functions: storage, cell transport, structure, catalysis
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lipids
- made up of macromolecules? - function: long-term energy storage
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nucleic acids
- made up of nucleotides - 2 types: DNA and RNA
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what is pH?
the measure of the concentration of hydrogen ions
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pH scale
- ranges from 0-14 - lower the number, the more H+ ions in a solution - 0-6 are acids; acids donate H+ ions into solutions - 7 is neutral - 8-14 are bases; bases accept H+ ions - it's 10x the number on the scale (so pH of 3 is 10x more acidic than a pH of 4 and a pH of 3 is 100x more acidic than a pH of 5)
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6 requirements of living organisms
1. every organism is composed of one or more cells. 2. living organism can autonomously reproduce. composed of DNA (genetic material that contains all of the instructions to build, maintain, and GROW) 3. living organisms must obtain energy from their environment 4. living organisms sense their environment and respond to it (respond to stimuli) 5. living organisms maintain internal conditions; homeostasis 6. groups of living organisms can evolve
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what is the smallest unit of life?
cell
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why are cells so small?
- a large surface area to volume ratio is important - more surface area you have \= more space for nutrients to go in and waste to go out
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cell theory (2 parts)
1. every living organism is composed of one or more cells 2. all cells living today came from preexisting cells
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prokaryotes
- NO nucleus - single celled - bacteria and archaea (bacteria do have ribosomes) - on average, much smaller than eukaryotic cells - most have a tough cell wall - lack membrane bound organelles (ribosomes present in cytoplasm) - usually have cilia, flagellum, or both
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eukaryotes
- ALL of these have a nucleus - internal complex compartments (membrane bound organelles) - can be BOTH single celled or multicellular
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what does eukarya mean?
"true nucleus"
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active transport
- movement of substances across a membrane requiring energy (ATP) - energy IS required
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passive transport
- movement of substances across a membrane without addition of energy - NO energy is required
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cytoplasm
- contains a thick fluid called cytosol, consisting of ions and biomolecules mixed in water - cytosol + organelles \= cytoplasm - would be air of the city
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nucleus
- contains DNA - think of it like the courthouse/city hall building, important documents are kept there
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nucleolus
- site of RNA used later by the cell - it's the copy machine (RNA is a copy of DNA since DNA can't leave nucleus)
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endoplasmic reticulum (er)
- interconnected network of tubes and flattened sacs that produces certain lipids and proteins
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rough er
- dotted with ribosomes that produce proteins for use both inside and outside the cell - factory where proteins are made
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smooth er
- produce lipids for other cellular compartments and help break down toxic organic compounds in the cell - think of it as the factory that synthesizes lipids and carbs
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Golgi apparatus
- directs proteins and lipids produced by ER to their final destination - packages and ships proteins, carbs, lipids to where they need to go - it's like the post office
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mitochondria
- extracts energy from food molecules, releasing carbon dioxide and water in an oxygen-dependent process called cellular respiration - ATP powerhouse - it's the power station
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cytoskeleton
- framework and structure/skeleton of buildings
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lysosomes
- only in animals - breaks down organic molecules when you're done with them - it's the trash can
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flagella
- only in animals - aids in swimming/movement - sperm
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central vacuole
- only in plants - holds water - water tower - chloroplasts
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cell wall
- to give structure and support - only in plants
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chloroplasts
- only in plants - capture energy from sunlight to make sugars (glucose) - does photosynthesis
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what organelles are only found in plants?
cell wall, chloroplasts, central vacuole
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what organelles are only found in animals?
flagella, lysosomes
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energy
the transfer of electrons and is important for life
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first law of thermodynamics
energy can neither be created nor destroyed, but can be converted from one form to another
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what is metabolism?
- the chemical reactions occurring inside a living organism - ability to capture, store, and use energy
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two types of metabolism
1. catabolism 2. anabolism
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catabolism
energy producing reactions that release chemical energy in the process of breaking down complex biomolecules - breaking bonds, releasing energy
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anabolism
energy requiring reactions that create complex biomolecules from smaller organic compounds - building bonds, investing/creating energy
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what is the most abundant energy carrying molecule?
ATP
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enzymes are what organic molecule?
proteins
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enzymes are catalysts. what does that mean?
they speed up chemical reactions
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how do enzymes speed up reactions?
by lowering the amount of energy necessary for the reaction to run - producing more product
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enzymes are substate specific. (specificity). what does that mean?
each enzyme matches a specific reaction; these are certain shapes that the 3D enzymes fit in to
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enzymes are 3D shapes. the location that the substrate binds to the enzyme is called the?
active site
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enzymes are reusable unless
denatured
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denaturation
the destruction of a protein's shape, resulting in a loss of protein activity
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when an enzyme loses its function it is said to be denatured. what are 3 things that change an enzyme's shape?
1. extreme temps 2. pH outside of optimal range 3. change in salt concentration (salinity)
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what is a metabolic pathway?
- chains of linked chemical reactions - produce key biological molecules in a cell - break down key biological molecules in a cell - 2 important types: photosynthesis and cellular respiration
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what is photosynthesis?
taking light energy and turning it into chemical energy (glucose)
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where does photosynthesis take place?
chloroplast
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who does photosynthesis?
plants and some bacteria
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2 stages of photosynthesis
1. light reaction 2. Calvin cycle
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light reaction
- needs light and water - light splits water molecule into H+ and O which generate energy carries NADPH and ATP - oxygen isn't needed so it's released through a pore in the leaf called the stomata - whole purpose of light reaction is to run dark reaction/Calvin cycle
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Calvin cycle
- energy from light reaction is used to run a series of enzyme catalyzed chemical reactions to convert carbon dioxide into sugar - does not require light - whole purpose is to convert carbon dioxide into sugar (glucose)
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What are the reactants of photosynthesis?
carbon dioxide and water
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What are the products of photosynthesis?
glucose and oxygen
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what is cellular respiration?
- the breakdown of food molecules to release a form of energy that's usable within a cell