Cloning

Describe micropropagation

• use forceps to remove plants

• wash in bleach

• grow on sterile agar in sterile tubes

• with a nutrient medium consisting of nitrates, amino acids, glucose

• add acid/alkali use SPECIFIC PH

• use several explants (cutting of donor)

• control TEMPERATURE, CARBON DIOXIDE, LIGHT

• use the same age plant

• count number of shoots after stated time

h

• nucleus from body cell

• inserted into enucleated egg cell

• electric shock

• triggers mitosis

• embryo

• implantation into surrogate female

e

m