Elisa

what does ELISA stand for?

Enzyme Linked ImmunoSorbent Assay

What does a direct ELISA detect

Detects antigen

How does direct ELISA work to detect specific antigen?

Antibody can bind specifically to antigen, antibody contains an enzyme that acts as a substrate and turns colored

What is a positive direct ELISA look like, and what does it mean?

Color change, this indicates the antibody which is super specific and has memory bind to the antigen, indicating that that is the specific antigen

What does a negative direct ELISA test look like and what does that mean?

No color change, meaning antibody doesn’t bind to antigen

What does an indirect ELISA detect for?

It detects if you have a specific antibody, indicates if you have previously exposed to pathogen before indirectly

How is an indirect ELISA set up

Assay contains antigen that is known to bind to specific antibody, another antibody containing enzyme will bind to primary antibody causing a color if detected

What is a positive indirect antibody test?

Color, meaning antibody does bind to antigen indicating previous exposure

What does a negative indirect antibody look like and what does it mean?

No color after adding antibody containing enzyme, meaning primary antibody didnt bind to antigen

Why is sandwhich ELISA used?

For more accuracy, more customization

Mainly used for antigens that are hard to purify and need stabilization and shit

How is a sandwich ELISA set up

Array contains antibody that is known to bind to angtigen

Second antibodyd containing enzyme acts as signal