Lab: Electrophoresis I

Micropipette

A tool for accurately measuring and transferring tiny

liquid volumes, essential for loading samples into gel wells.

Agarose Gel

A jelly-like substance that acts as a sieve, allowing

molecules to separate by size as they move through it during

electrophoresis.

Electrophoresis

A technique for separating molecules (like DNA) by

size and charge, where an electric field pushes them through an agarose gel.

Power Supply

Supplies the electric current that drives molecules

through the gel; it controls the speed and distance of movement.

Electrophoresis Chamber

A container holding the gel and buffer,

connected to the power supply. It has electrodes to create the electric field.

Polarity

Refers to the positive (+) and negative (−) ends in the electric

field. DNA moves toward the positive end due to its negative charge.