DNA extraction delta

1. Homogenization

2. Lysis.

3. Precipitation.

4. Purification.

identify the step ?

why are we doing it ?

homogenization .

Breaking the cells to open them to expose DNA. This known as cell disruption

what material is needed in this step ?

tissue buffer

ovaries or testes of dog , cat or rabbit

cold mortar and a pestle

SDS (sodium dodecyl sulfate)

identify the step ?

why we do it ?

lysis

dissolve cellular membranes and remove contaminants

what material is needed in this step ?

centrifuge tube

extraction buffer

identify the step ?

why do we do it ?

precipitation

separates DNA from this cellular debris.

what material is needed in this step ?

Na + ions (sodium ions)

alcohol

centrifuge device

identify the step ?

why do we do it ?

purification

get the extracted DNA in the most pure form

what material is needed in this step ?

a new centrifuge tube

pasture pipette

alcohol (ethanol)

glass rod