SDS-Page Gel

Rf value

it passes through a system where the smaller molecules can move more freely than the larger molecules the detergent gives them a negative charge as they pass through

Gel filtration chromatogrpahy

seperates based on size, configuration, olgiometric state

In SDS -PAGE

runs towards positive smaller Rf

Comassie stain

nonspecific whereas Western blot is specific proteins because of antibodies