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light microscope
100x magnification, visible light passes through the specimen and lenses enlarge the image. can be used to view living cells.
Electron Microscope
1000x magnification, Uses beams of electrons to resolve objects,Has a higher resolution, and able to see cellular organelles
Scanning electron microscopes
(SEMs) examine cell surfaces
Transmission electron microscopes
(TEMs)are useful for
studying the internal structure of a cell.
Cell fractionation
takes cells apart and separates the major organelles from one another
hydrolysis
the chemical breakdown of a compound due to reaction with water.
prokaryotic cells
are older than eukaryotic cells, usually smaller, simpler in structure, have no nucleus, DNA is coiled, cell wall with slime layer, have flagellum
eukaryotic cells
have organelles, have a nucleus that has the DNA, they have cytoplasm
cytoplasm
The region between the nucleus and plasma membrane is the___. It consists of various organelles suspended in the liquid cytosol
cytosol
the fluid present in the cell and is a constituent of the cytoplasm
Ribosomes
are constructed from a type of RNA, and use the information from the DNA to
make proteins, carry out protein synthesis
plasma membrane
is a selective barrier that allows sufficient passage of oxygen, nutrients, and waste to service the volume of every cell
phospholipid bilayer
has the phospho lipid which is a head and two fatty acid tails, which is hydrophobic and hydrophilic.helping with liquids in the cell.
nucleoid
a region of a prokaryotic cell that contains the bacterial chromosome.
organelles
internal membranes that divide the cell into compartments, perform specific functions (divide labor), and isolate reactions from others
endomembrane system
Organelles that are connected and communicate with one another, Directly via membrane channels, and Indirectly via small transport vesicles
chloroplasts
which convert light energy to the chemical energy of food in the process of photosynthesis, found only in plants and algae
Mitochondria
are the sites of cellular respiration, a metabolic process that uses oxygen to generate ATP, are found in BOTH plant and animal cells
Nuclear Envelope
The nucleus is separated from the cytoplasm by a double membrane called the____. Pores in the envelope allow materials to move between the nucleus and cytoplasm.
nucleolus
The nucleus contains a dark region in the center called the___, where ribosomes are made.
Ribosomal RNA
Ribosomes are constructed from a type of RNA called___. They help aid in making of proteins
Chromosomes
In the nucleus, DNA is organized into discrete units called
Chromatin
Each chromosome contains one DNA molecule associated with proteins, called___. It condenses to form discrete chromosomes as a cell prepares to divide.
vesicle
helps with things such as helping to transport materials that an organism needs to survive
Rough ER
whose surface is studded with ribosomes, Studded with bound ribosomes that synthesize secretory proteins including glycoproteins, Secretory proteins are bound for secretion from the cell, Glycoproteins are proteins covalently bonded to carbohydrates, Distributes secretory proteins in transport vesicles, and Is a membrane factory for the cell
Smooth ER
No bound ribosomes, Site of lipid synthesis, Enzymes synthesize oils, steroids, and membrane phospholipids, Detoxifies drugs and poisons (especially in liver cells), Stores calcium ions (in muscle cells), and r eleases of stored calcium ions can trigger muscle contractions
cisternae
packaging, modification, and transport functions for the cell overall
glycoproteins
are proteins covalently bonded to carbohydrates
lysosome
is a membranous sac of hydrolytic enzymes that can digest macromolecules
phagocytosis
The process by which a phagocyte (a type of white blood cell) surrounds and destroys foreign substances (such as bacteria) and removes dead cells
food vacuoles
are formed by phagocytosis, a membrane-enclosed sac, which has a digestive function
Contractile vacuoles
found in many freshwater protists, pump excess water out of cells
peroxisome
are oxidative organelles, are specialized metabolic compartments bounded by a single membrane, found in both plant and animal cells, perform reactions with many different functions, reactions produce hydrogen peroxide and convert it to water using special enzymes
endosymbiont theory
suggests that an early ancestor of eukaryotes engulfed an oxygen-using non-photosynthetic prokaryotic cell, The engulfed cell formed a relationship with the host cell, becoming an ___.
chlorophyll
Chloroplasts contain the green pigment ___.
cytoskeleton
is a network of fibers that organizes structures and activities in the cell, is dynamic, and contribute to the amoeboid (crawling) movements of, the protist Amoeba and some of our white blood cells.
microtubals
are straight and hollow tubes that maintain cell shape and guide the movement of organelles and chromosomes, interact with motor proteins to produce cell motility (as in cilia or flagella), and separate chromosomes during cell division
intermediate filaments and micro filaments
are thinner and solid fibers that maintain cell shape, change cell shape, and are involved in muscle contraction.
centrosome
near the nucleus, has a pair of centrioles, each with nine triplets of microtubules arranged in a ring.
centrioles
organizing microtubules that serve as the cell's skeletal system, an organelle that assists cell division, allowing cells to replicate
flagella
A core of microtubules sheathed by the plasma membrane, have an undulating motion like the tail of a fish, allows a cell to move
cilia
microtubule-containing extensions that project from some cells, have a back and forth motion, and A core of microtubules sheathed by the plasma membrane, hair-like structures, also to help move and attach
basal body
that anchors the cilium or flagellum
cell wall
is an extracellular structure that distinguishes plant cells from animal cells, · surround the membrane and are made of cellulose, protect the cells, maintain cell shape, and keep cells from absorbing too much water.
plasmodesmata
microscopic channels which traverse the cell walls of plant cells and some algal cells, enabling transport and communication between themnels between adjacent plant cells,
Extracellular Matrix
(ECM) Animal cells lack cell walls but are covered by an elaborate___. made up of glycoproteins such as collagen, proteoglycans, and fibronectin, hold cells together in tissues and protects and supports them, proteins bind to receptor proteins in the plasma membrane called integrins, can regulate a cell’s behavior by communicating with a cell through integrins, around a cell can influence the activity of gene in the nucleus, and Mechanical signaling may occur through cytoskeletal changes, that trigger chemical signals in the cell
tight junctions
membranes of neighboring cells are pressed together, preventing leakage of extracellular fluid
Desmosomes
(anchoring junctions) fasten cells together into strong sheets
Gap Junctions
(communicating junctions) provide cytoplasmic channels between adjacent cells
amphipathic
a chemical compound containing both polar (water-soluble) and nonpolar (not water-soluble) portions in its structure.
fluid mosaic model
a membrane is a fluid structure with a “mosaic” of various proteins embedded in it
Cholesterol
content affects membrane fluidity, lives as a buffer for the bilayer
peripheral proteins
are bound to the surface of the membrane, a protein that is found temporarily attached to the cell or mitochondrial membrane
integral proteins
penetrate the hydrophobic core, a type of membrane protein that is permanently attached to the biological membrane.
transmembrane proteins
Integral proteins that span the membrane are called
glycolipids
a lipids that has to maintain the stability of the cell membrane and to facilitate cellular recognition
glycoproteins
are formed when the protein component predominates in the combination of carbohydrates and proteins.
selectively permeable
DEFINITION Allows some substances to move across the membrane. chooses when and when not too.
Transport proteins
proteins that move molecules across a membrane within a cell, include channel proteins and carrier proteins
aquaporins
facilitate the passage of water through and in a cell
concentration gradient
the region along which the density of a chemical substance increases or decreases
diffusion
the natural movement of particles from an area of higher concentration to an area of lower concentration due to random molecular motion
passive transport
is the diffusion of a substance across a membrane WITHOUT the input of energy.
facilitated diffusion
The passive movement of molecules down their concentration gradient with the help of transport proteins is called
osmosis
The diffusion of water across a selectively permeable membrane is
tonicity
is the ability of a surrounding solution to cause a cell to gain or lose water
isotonic solution
has an equal concentration of solute as that inside a cell
hypertonic solution
has a higher concentration of solute than that inside the cell
hypotonic solution
has a lower concentration of solute than that inside the cell
plasmolysis
When a plant cell is immersed in a hypertonic solution, the plant cell loses water, shrivels and the plasma membrane pulls away from the cell wall
osmoregulation
is the control of water balance within a cell or organism.,
The balance of water and solutes outside the cell MUST EQUAL the balance of water and solutes inside the cell, Plant and animal cells maintain
active transport
requires that a cell expend energy to move molecules across a membrane.
uses energy to move solutes against their gradients
membrane potential
is the voltage difference across a membrane, is a source of potential energy for the cell, acts like a battery (an energy source)
electrochemical gradient
Two combined forces, collectively called the _______, drive the diffusion of ions across a membrane
electrogenic pumps
Special transport proteins, called ______, generate the voltage gradient across a membrane. · help store energy that can be tapped for cellular work.
proton pump
· In plants, bacteria, and fungi, a ____ is the major electrogenic pump, actively transporting protons out of the cell and transferring positive charge from the cytoplasm to the extracellular solution.
cotransport
occurs when active transport of a solute indirectly drives transport of other substances. As the solute that has been actively transported across the membrane is allowed to passively diffuse back through a transport protein, its movement can be coupled with the active transport of another substance AGAINST its concentration (or electrochemical) gradient. Plants commonly use the gradient of hydrogen ions generated by proton pumps to drive active transport of nutrients into the cell.
exocytosis
transport vesicles migrate to the membrane, fuse with it, and release their contents outside the cell. Bulk transport across the plasma membrane occurs by___. Many secretory cells use___.
endocytosis
the cell takes in macromolecules by forming vesicles from the plasma membrane. is essentially a reversal of exocytosis.
pinocytosis
(“cellular drinking”), Vesicles form around bulk quantities of liquid or very small particles
receptor mediated endocytosis
a means to import macromolecules from the extracellular fluid, absorb metabolites, hormones, proteins – and in some cases viruses – by the inward budding of the plasma membrane
metabolism
transforms matter and energy, subject to the laws of thermodynamics, The sum of all the chemical reactions that occur in the body, Reactants participate in a reaction, Products form as a result of a reaction
metabolic pathway
Reactions usually occur in a sequence forming a ____, Begins with a particular reactant, Proceeds through several intermediates, Terminates with a particular end product, Each step is catalyzed by a specific enzyme
anabolic pathway
Larger, complex molecules are made from simpler compound, Requires energy
catabolic pathway
Larger, complex molecules are broken down into simpler compounds, Releases energy, Hydrolysis Reactions, Used to decompose/degrade macromolecules into subunits, Water is used to split the substances, Can provide energy for the cell, Metabolic reactions are often reversible
energy
The ability to do work or bring about a change. kinetic, potential, thermal, chemical. cannot be created or destroyed but it can be transferred from one form to another.
kinetic energy
Energy of motion (mechanical energy)
potential energy
Stored energy, Based on matter’s location or structure
thermal energy
is kinetic energy associated with random movement of atoms or molecules, _____in transfer from one object to another is called heat.
chemical energy
is potential energy available for release in a chemical reaction, Energy cannot be created or destroyed but it can be transferred from one form to another.
thermodynamics
is the study of energy transformations
entropy
is the measure of disorder or randomness, The dispersal of heat/energy in a system.
free energy
The amount of energy available to perform work
exergonic reactions
Products have less free energy than reactants, Spontaneous, releases energy
endergonic reactions
Products have more free energy than reactants, Not spontaneous, requires energy
ATP
adenosine triphosphate,
High energy compound used to drive metabolic reactions, Constantly being generated from adenosine diphosphate (ADP), Composed of Adenine, ribose (together = adenosine), and three phosphate groups
enzymes
Protein molecules that function as catalysts, Speed up reactions, Each enzyme accelerates a specific reaction, Each reaction in a metabolic pathway requires a unique and specific enzyme, The end product will not be formed unless ALL enzymes in the pathway are present and functional
catalyst
a chemical that speeds up chemical reactions.
activation energy
is often supplied in the form of thermal energy that the reactant molecules absorb from their surroundings, The initial energy needed to start a chemical reaction is called the free energy of activation