Exam 3 photos

Masson trichrome (1)

Collagen, muscle, fibrin

Masson trichrome (3)

Collagen, muscle, fibrin

Masson trichrome

Weak nuclear stain

Masson Trichrome
Overstained with iron hematoxylin

Masson Trichrome
Weak muscle staining

• Overtain w/iron hem

• bad reagents

• No pretreatment w/Bouins

Masson Trichrome

Weak Biebrich staining and grey muscle

Masson trichrome

Weak aniline blue staining

Masson Trichrome

Absent aniline blue staining

Gomori Trichrome

Collagen, muscle, fibrin

Gomori Trichrome (3)

Collagen, muscle, fibrin

VVG (skin)

Elastic fibers

VVG (Lung)

Elastic fibers

VVG (2)

Elastic fibers

VVG (vessel) - overdifferentiated w/ferric chloride

VVG (lung?) - overdifferentiated w/ferric chloride

VVG (lung) - underdifferentiated

VVG (GI) - underdifferentiated

VVG (lung) - overcounterstained w/Van Gieson

Aldehyde Fuchsin

Elastic fibers

Orcein

Elastic fibers

Orcein close up

elastic fibers

Resorcin-fuchsin w/NFR counterstain

Elastic fibers

Movat pentachrome

Mucin, fibrin, elastin, muscle, collagen

Movat pentachrome on Temporal Artery

Mucin, fibrin, elastin, muscle, collagen

Movat pentachrome

Weak yellow staining

Gomori Reticulin (green)

Reticular fibers

Gomori Reticulin

Reticular fibers

Background staining around section

• Improperly cleaned supplies

• Used metal tools or fixative

• pH of impregnating solution lower than 11, tap water used

• No rinsing after silver impregnation or Not enough time in hypo (which removes unreduced silver)

• Waterbath adhesives

Gomori reticulin Overstained

• Prolonged exposure in sensitizer (ferric ammonium sulfate)

Gomori Reticulin (1) - Incomplete impregnation

• Silver solution old

• Old/weak OH, or too much OH

• Not enough time in silver soln

• Prolonged rinsing after silver impregnation

Gomori Reticulin (2) - Incomplete impregnation

• Silver solution old

• Old/weak OH, or too much OH

• Not enough time in silver soln

• Prolonged rinsing after silver impregnation

Gomori Reticulin - Weak staining

Gomori reticulin - overtoned
Looks purple

• Too much time in toner → Must place slides in Na metabisulfate. Hypo can also help

PA-MS

Basement membrane

PA-MS - Weak staining

PA-MS - Overstained

• Overstained with silver solution

PA-MS — Overcounterstained

PA-MS — with an H&E counterstain

PTAH

Cross-striations and fibrin

PTAH (darker)

Cross striations and fibrin

PTAH for fibrin in glomerular thrombosis

(kidney)

MSB (Lendrum)

Fibrin stages (new = yellow, middle = red, old = blue)

MSB (Lendrum) in a fibrin thrombus (mostly blue)

Fibrin stages (new = yellow, middle = red, old = blue)

Toluidine Blue

Mast cells

Toluidine blue — Overstained

Oil Red O

Simple lipids only

Oil red o when too much pressure during coverslipping

Sudan Black B

Simple and compound

Osmium tetroxide

Simple and compound lipids

Cresyl Echt/Fast Violet

• Nissl Substance

Bodian/Palmgren AKA Holmes w/o counterstain

• Nerve fibers

Bodian/Palmgren AKA Holmes w/ aniline blue counterstain

• Nerve fibers

Bielschowsky

• Nerve fibers AND neurofibrils

Bielschowsky (more stringy)

• Nerve fibers AND neurofibrils/neurofibril tangles

Bielschowsky-PAS

• Nerve fibers AND neurofibrils

Thioflavin S

• Nerve fibers and neurofibrils

• Senile plaques

PTAH (fibrous astrocytes)

• Glial cells and myelin

PTAH (with pink in center)

• Glial cells and myelin

Holzer (with astrocytes)

• Glial cells

Weil method

• Myelin (compound lipids)

** Note that this is a regressive method

Weil method (2)

• Myelin (compound lipids)

LFB

• Myelin (compound lipids)

LFB and Hematoxylin

• Myelin (compound lipids)

LFB and Cresyl violet

• Myelin (compound lipids)

LFB and PAS

• Myelin (compound lipids)

• Basement membranes

• Senile plaques, fungus