3.2: catabolic core of metabolism

1. To discuss how the link reaction and citric acid cycle are regulated, reflecting the need for ATP and use of NADH, through allosteric effects on key enzymes. 2. To explain the functioning of the electron transport chain in creating a proton gradient over the inner mitochondrial membrane, how the proton gradient drives ATP synthesis, and how uncoupling agents play a role in thermogenesis.

What is the link reaction?

The irreversible reaction of turning pyruvate into acetyl CoA, catalysed by pyruvate dehydrogenase.

Explain what happens in the Krebs cycle.

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