ISBB Part 5

What is the common type of autologous donation

Preoperative Collection (Predeposit)

Autologous Donation: Collected at the start of operation

Acute Normovolemic Hemodilution

What is the procedure called wherein Px collected blood will be replaced with saline prior to Sx

Acute Normovolemic Hemodilution

Blood that you lose will be collected, after the operation it transfuse

Intraoperative Collection

Not advisable type of autologous donation

Postoperative Collection

Autologous Donation Requirements

Age

no age requirement

Autologous Donation Requirements

Hemoglobin

at least 11g/dL

Autologous Donation Requirements

Hematocrit

at least 33%

Type of donation that means to seperate or to remove

Apheresis Donation

Type of Apheresis that checks patient platelets count

Plateletpheresis

What is the req. platelet count for plateletpheresis

< 150,000/UL

Type of pheresis that uses HES or Hydroxyethyl Starch

Leukapheresis

What is the purpose of HES

RBC sedimenting agent

This type of pheresis that can collect up to 2 units from the donor

Erythrocytapheresis

What is the adjustment period for the body in autologous donation

3 days

What is the adjustment period for the body in allogenic donation

3 months

How many days before the surgery should the final phlebotomy in autologous donation

3 days

What are the four pre-transfusion compatibility tests

- ABO typing

- RH typing

- AB screen

- Crossmatching

What is the product of A gene

n-acetyl galactosaminyl

What is the predominant sugar of A gene

n-acetyl galactosamine

What is the product of B gene

galactosyl transferase

What is the predominant sugar of B gene

galactose

What is the product O gene

none

What is the predominant sugar of O gene

L-fucose

ABO type that reacts most with anti-H lectin

Type O

ABO discrepancy

weak or missing antibodies

Group 1

ABO discrepancy

weak or missing antigen

Group 2

ABO discrepancy

plasma proteins

Group 3

ABO discrepancy

miscellaneous causes

Group 4

What are the RH genes

RhD and RhCE

Location of RH genes

Chromosome 1

What are the RH antigens

D, C, E, c, e

What is the purpose of the RH typing

detects for D antigen

What is the purpose of the weak D testing

differentiate weak D from RH-negative

Causes of Weak D (3)

1. Genetic

2. C-trans

3. Partial D/ Mosaic D

What is the reagent for weak D typing: What must be done before adding the reagent

AHG; wash 3 times with NSS

What is the purpose of washing

To remove free or unbound antibodies

What is the needle size for transfusion

18g

What is the needle size for donation or collection

16g

What IV fluids can be used during transfusion (2); What is the most preferred

NSS and 5% NSS

Blood warming devices should be set at what temperature

37degC

Slow Infusion

first 10-15 minutes or 2mL/min

Transfusion must be completed within how many hours

4 Hours

The blood should not be away from the BB for more than

30 minutes

Transfusion Reaction:

-Abs that activate complement

Immediate HTR

Transfusion Reaction:

-Abs that cannot activate complement

Delayed HTR

Transfusion Reaction:

-caused by WBC

- Leukoreduced should be given

-Febrile non-hemolytic trans reac

-NCPE / TRALI

Transfusion Reaction:

-the plasma should be removed

- Washed component should be given

Allergic (urticarial) trans reac

Transfusion Reaction:

-Washed component should be given

- IgA should be removed

Anaphylactic trans reac

Transfusion Reaction:

- iatrogenic causes

What should be the intervention?

TACO (trans-assoc circulatory overload)

If konti pa lang - IV diuretics

If marami na - therapeutic phleb

Transfusion Reaction:

- caused by storage lesions

- FIFO

Physically or Chemically-induced trans reac

Transfusion Reaction:

-clots/hemolysis/murky appearance

Bacterial Contamination

Most common organism assoc with blood bag contamination

Yersinia enterocolitica

Transfusion Reaction:

Irradiated components should be given

Transfusion assoc Graft vs Host Disease

When should spx for billirubin collected?

3-6 hours after transfusion