bacterial growth

how do bacteria replicate

binary fission

whats a single generation of growth

from 1 cell to 2 cells

whats an arathmatic scasle

each diviso has the same numerical vlaue

whats a logarithmic scale

dont increace by units of 1 but each section increaces to the power of 10

how do you calculate the final cell number (N)

N=N02n

what does N0 stand for

initial cell number

what does the 2 n stand for

number of generations during period of exponential growth

what are the 4 parts of bacteria growth

lag, exponential, stationary and death

describe the lag phase

culture is innoculated in fresh media and theres growth dependant on the enviromental conditions

whats the exponential stage

cell population doubles at regular intervals, healthiest stage

describe the exponential phase

the essential nutrient in the culture medium runs out and organism waste product builds to toxic levels - net growth and death

describe the death phase

exponential decline of cells

what are the 3 main ways to mesure cell growth

microscopic counts, viable cell counts and spectrophotomery

describe using microscopes to count cells

count the number of cells present e.g on a dry sample or a counting chamber for liquid cultures

describe viable cell counts to record bacteria

reverse serial dilution

describe spectrophotonomy to count cells

the more cells the more scatterd light therefore the more scatterd light the more organisms