BACTE LAB MOCK EXAM

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THIS IS A TYPE OF BSC THAT IS THE LEAST EFFECTIVE AS IT IS ENTIRELY OPEN

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1

THIS IS A TYPE OF BSC THAT IS THE LEAST EFFECTIVE AS IT IS ENTIRELY OPEN

CLASS I

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2

THIS IS A TYPE OF BSC THAT IS THE MOST USED IN LABORATORIES

CLASS II/LAMINAR FLOW

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3

A SUBCLASS OF CLASS II BIOSAFETY CABINET THAT EXHAUST AIR INSIDE THE ROOM

IIA

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4

A SUBCLASS OF CLASS II BIOSAFETY CABINET THAT EXHAUST AIR OUTSIDE THE ROOM

IIB

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5

THIS IS THE MOST EFFECTIVE OF ALL CLASSES WHEREIN IT HAS A PART WHERE OBJECTS MAY PASS THROUGH

CLASS III

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6

THIS BSC HAS BUILT IN GLOVES, SEALED TO THE CABINET

CLASS III

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7

WHAT ARE BACTERIA/VIRUSES THAT ARE UNDER BSL-1

BACILLUS SUBTILIS AND MYCOBACTERIUM GORDONAE

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8

WHAT ARE BACTERIA/VIRUSES THAT ARE UNDER BSL-2

SALMONELLA, SHIGELLA, STAPHYLOCOCCUS AUREUS, HIV, HBV

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9

WHAT ARE BACTERIA/VIRUSES THAT ARE UNDER BSL-4

ARENAVIRUS, MARGBURG VIRUS

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10

BSL-2 ORGANISMS ARE ACQUIRED THROUGH?

INGESTION, MUCUS MEMBRANE, AND PERCUTANEOUS EXPOSURE

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11

WHAT ARE BACTERIA/VIRUSES THAT ARE UNDER THE CATEGORY A BIOTERRORISM?

SMALLPOX VIRUS, FRANCISCELLA, BACILLUS ANTHRACIS

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12

WHAT ARE BACTERIA/VIRUSES THAT ARE UNDER THE CATEGORY B BIOTERRORISM?

RICKETTSIA, COXIELLA, BURKHOLDERIA PSEUDOMALLEI

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13

WHAT ARE BACTERIA/VIRUSES THAT ARE UNDER THE CATEGORY C BIOTERRORISM?

YELLOW FEVER, VIRUS, DENGUE, EBOLA VIRUS, MERS-COV, ZIKA VIRUS

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14

WHAT IS THE AGENT OF BLACK PLAGUE?

YERSINIA PESTIS

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15

PRESENCE OF VIABLE BACTERIA IN THE BLOOD

BACTEREMIA

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16

THIS IS A TYPE OF BACTEREMIA ACCOMPANIED BY SYSTEMIC INFLAMMATORY REPSONSE

SEPTICEMIA

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17

WHAT ARE THE COMMON CONTAMINANTS IN BLOOD

S. EPIDERMIDIS, VIRIDANS STREPTOCOCCI, PROPIONIBACTERIUM ACNES, BACILLUS SPECIES, DIPHTHEROIDS

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18

NEW NAME OF PROPIONIBACTERIUM ACNES?

CUTIBACTERIUM ACNES

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19

ALL BACILLUS SPECIES ARE A COMMON BLOOD CONTAMINANT EXCEPT FOR?

BACILLUS ANTHRACIS

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20

ANTICOAGULANT USED FOR BLOOD CULTURE

0.025% SPS

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21

SPS IS AN INHIBITORY FOR?

NEISSERIA, PEPTOSTREPTOCOCCUS ANAEROBIUS, GARDNERELLA VAGINALIS, STREPTOBACILLUS MONILIFORMIS

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22

REMEDY TO NEUTRALIZE THE EFFECT OF SPS

0.1% GELATIN

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23

OTHER ADDITIVE USED FOR BLOOD CULTURE

SODIUM AMYLOSULFATE, 0.5-1.0% SODIUM CITRATE, HEPARIN

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24

WHAT ADDITIVE IS USED TO RECOVER VIRUS IN THE BLOOD, CAN INHIBIT THE GROWTH OF SOME GRAM NEGATIVE BACTERIA AND NOT SUITABLE FOR THE RECOVERY OF FUNGI AS IT CAN INHIBIT YEAST

HEPARIN

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25

THIS ADDITIVE IS NEVER USED IN BLOOD CULTURE

EDTA

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26

MEDIA USED FOR BLOOD CULTURE

TRYPTICASE SOY AGAR, BRAIN HEART INFUSION, BRUCELLA BROTH

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27

INDICATIONS FOR POSITIVE GROWTH IN THE BROTH INCLUDES PRESENCE OF

HEMOLYSIS, PELLICLE, TURBIDITY

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28

BLOOD CULTURE IS SUBCULTURE IN THREE CULTURE MEDIA

CHOCOLATE AGAR PLATE, BLOOD AGAR PLATE, MACCONKEY AGAR PLATE

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29

POSITIVE GROWTH WITHIN 1-3 DAYS IN SUB CULTURE MEDIA MEANS?

PATHOGENIC

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30

POSITIVE GROWTH IN 5TH DAYS IN SUB CULTURE MEDIA MEANS?

CONTAMINANT

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31

WHAT SPECIMEN IS USED TO DERECR TUBERCULOSIS AND PNEUMONIA

SPUTUM

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32

WHAT BACTERIA IS PRESENT IN SPUTUM SPECIMEN?

MYCOBACTERIUM TUBERCULOSIS

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33

WHAT BSL IS USED AS WELL AS BSC IN M. TUBERCULOSIS

BSL-3; BSC II

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AGENTS OF PNEUMONIA

KLEBSIELLA PNEUMONIAE, STAPHYLOCOCCUS AURUES, PSEUDOMONAS AERUGINOSA

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AGENTS FOR VENTILATOR-ASSOCIATED PNEUMONIA

STREPTOCOCCUS PNEUMONIAE, HAEMOPHILUS INFLUENZAE, STAPHYLOCOCCUS AUREUS, KLEBSIELLA ENTEROBACTER, SERRATIA

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MEDIA USED FOR M. TUBERCULOSIS

LOWENSTEIN-JENSEN MEDIA AND MIDDLBROOKE 7H9 AND 7H10 BROTH

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NO GROWTH IN LJ MEDIA AT 37 DEG WITHIN 8 WEEKS MEANS?

(-) MYCOBACTERIUM TUBERCULOSIS

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(+) GROWTH IN LJ MEDIA AT 37 DEG WITHIN 2-3 WEEKS MEANS?

(+) MYCOBACTERIUM TUBERCULOSIS

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39

WHAT IS THE APPEARANCE OF COLONIES IN M. TUBERCULOSIS HAVE IN LJ MEDIA

CAULIFLOWER LIKE

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WHAT MEDIA IS USED TO RECOVER S. PNEUMONIAE

GENTAMICIN BAP

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WHAT MEDIA IS USED TO RECOVER H. INFLUENZAE

BACITRACIN BAP

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42

WHAT IS USED TO DETERMINE IF SAMPLE IS TRUE SPUTUM OR SALIVA

BARTLETT’S CLASSIFICATION

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43

WHAT IS COUNTED IN BARTLETT’S CLASSIFICATION

POLYMORPHONUCLEAR NEUTROPHILS AND SQUAMOUS EPITHELIAL CELLS

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ACCORDING TO BARTLETT’S CLASSIFICATION, WHAT IS TRUE SPUTUM?

PMN: >25 AND SEC: <10

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ACCORDING TO BARTLETT’S CLASSIFICATION, WHAT IS SALIVA?

PMN: <25 AND SEC: >10

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STORAGE TEMP FOR M. TUBERCULOSIS WHEN NO PROCESSED IMMEDIATELY

4 DEG CELSIUS (REFRIGERATOR TEMPERATURE)

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47

HOW IS SPUTUM COLLECTED?

DEEP COUGH

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48

RECOMMENDED VOLUME OF SPUTUM SPECIMEN FOR DIAGNOSIS OF M. TUBERCULOSIS

5-10 ML

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49

GOLD STANDARD FOR DIGESTION AND DECONTAMINANT FOR MYCOBACTERIUM TUBERCULOSIS

N-ACETYL-L-CYSTEINE AND 1% SODIUM HYDROXIDE

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50

LIQUEFY MUCOUS BY USING DIGESTANT/MUCOLYTIC AGENT

DIGESTION

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51

THIS REMOVES CONTAMINANTS AND NORMAL FLORA

DECONTAMINATION

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52

WHAT IS USED AS A DECONTAMINANT IF THE SPX IS LIKELY TO CONTAIN PSEUDOMONAS SPECIES

5-6% OXALIC ACID AND ZEPHIRAN-TRISODIUM PHOSPHATE

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53

WHAT SPECIMEN IS GENERALLY USED FOR AEROBIC BACTERIA AND LEAST PREFERRED FOR ANAEROBIC BACTERIA

SWABS

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54

MATERIALS FOR SWAB SPECIMEN

COTTON, DARCON, RAYON, CALCIUM ALGINATE

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55

WHAT IS TOXIC TO NEISSERIA?

COTTON

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56

THIS IS SAID TO BE TOXIC TO VIRUS

CALCIUM ALGINATE

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57

THIS MATERIAL FOR SWAB IS SUITABLE FOR BACTERIA AND VIRUS

DACRON/RAYON

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58

MAJOR THROAT PATHOGEN IS

STREPTOCOCCUS PYOGENES

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S. PYOGENES CAUSES _____ WHICH OCCURS AMONG ___

PHARYNGITIS/STREPTHROAT; CHILDREN

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WHICH BACTERIA IS KNOW TO HAVE A VIRULENCE FACTOR M PROTEIN

S. PYOGENES

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A VIRULENCE FACTOR THAT IS RESPONSIBLE FOR THE SEQUALAE INFECTION OF S. PYOGENES

M PROTEIN

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62

SEQUALAE INFECTION OF S. PYOGENES MAY LEAD TO?

RHEUMATOID HEART DISEASE AND ACUTE GLOMERULONEPHRITIS

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63

A TEST TO DETECT ID THE CAUSE OF SORE THROAT IS S. PYOGENES

ANTISTREPTOLYSIN O TEST

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64

WHAT IS THE MAJOR THROAT FLORA

VIRIDANS STREPTOCOCCI

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65

IN IMMUNOCOMPROMISED PXS, V. STREPTOCOCCI MAY CAUSE _____

SUB-ACUTE BACTERIAL ENDOCARDITIS

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66

BACTERIA FOR NASOPHARYNGEAL SWAB

BORDETELLA PERTUSSIS, NEISSERIA MENINGITIDIS, HAEMOPHILUS INFLUENZAE, MRSA, SARS-COV

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CAUSATIVE AGENT OF WHOOPING COUGH

BORDETELLA PERTUSSIS

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68

CAUSATIVE AGENT OF MENINGITIDIS

HAEMOPHILUS INFLUENZAE

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69

THIS IS THE NORMAL FLORA OF ORO/NASOPHARYNX

NEISSERIA MENINGITIDIS

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70

WHAT SPECIMEN IS USED FOR DIAGNOSING MENINGITIDIS

CSF

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71

THIS CAN CAUSE MENINGITIDIS AMONG 5-29 YEARS OLD

NEISSERIA MENINGITIDIS

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72

GENITAL SWAB IS USED FOR WHAT BACTERIA?

NEISSERIA GONORRHEAE

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73

STORAGE TEMP OF N. GONORRHEAE

ROOM TEMPERATURE

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74

WHICH SPECIMEN IS COLLECTED IF THE PATIENT IS INTUBATED OR IN A VENTILATOR

ENDOTRACHEAL ASPIRATE

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75

THIS SAMPLE IS THE TOP PRIORITY IN THE BACTERIOLOGY LABORATORY

CEREBROSPINAL FLUID

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MANNER OF COLLECTION OF CSF

LUMBAR TAP/SPINAL TAP

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COLLECTION OF CSF (IN ORDER TUBES)

CHEMISTRY, MICROBIOLOGY, HEMATOLOGY

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IN CASE ONLY ONE TUBE IS COLLECTED FOR CSF WHAT SHOULD BE THE ORDER OF DEPARTMENT

MICROBIOLOGY, HEMATOLOGY, CHEMISTRY

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79

STORAGE TEMP OF CSF

ROOM TEMPERATURE

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80

COMMON PATHOGENS IN CSF

CRYPTOCOCCUS NEOFORMANS, HAEMOPHILUS INFLUENZAE, NEISSERIA MENINGITIDIS, STREPTOCOCCUS AGALACTIAE, STREPTOCOCCUS PNEUMONIAE, LISTERIA MONOCYTOGENES

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THIS CAN BE FOUND IN CSF BUT IS NOT CONSIDERED AS A BACTERIA BUT RATHER A CAPSULATED YEAST

CRYPTOCOCCUS NEOFORMANS

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82

THIS IS USED TO VISUALIZE CRYTPCOCOCCUS NEOFORMANS

INDIA INK

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83

MEDIA FOR DIAGNOSING PATHOGENS IN CSF

BAP, CAP

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84

STOOL SPECIMEN IS USED FOR THE DETECTION OF WHAT PATHOGENS?

ENTERIC PATHOGENS

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85

TRUE OR FALSE. URINE SPECIMEN IS INOCULATED STRAIGHT IN A PLATED MEDIA

FALSE

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86

MEDIA FOR STOOL

SALMONELLA SHIGELLA AGAR, EOSIN METHYLENE BLUE, MCCONKEY AGAR

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87

COMMON PATHOGENS FOUND IN STOOL

CAMPYLOBACTER JEJUNI, SALMONELLA, SHIGELLA, ESCHERICHIA COLI, VIBRIO SPECIES, YERSINIA ENTROCOLITICA

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88

ALTERNATIVE SPECIMEN FOR STOOL

RECTAL SWAB

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89

MOST COMMON CAUSE OF UTI

ESCHERICHIA COLI

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90

COMMON CAUSE OF UTI AMONG SEXUALLY ACTIVE YOUN FEMALE

STAPHYLOCOCCUS SAPROPHYTICUS

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91

PREFERRED MANNER OF COLLECTION IN URINE AND IS SUITABLE FOR THE RECOVERY OF AEROBIC BACTERIA

MIDSTREAM CLEAN CATCH

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92

PREFERRED MANNER OF COLLECTION IN URINE FOR INFANTS AND IS SUITABLE FOR THE RECOVERY OF ANAEROBIC BACTERIA

SUPRAPUBIC ASPIRATION

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93

URINE SHOULD BE PROCESSED WITHIN?

1 HOUR

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94

IF URINE SPECIMEN IS NOT PROCESSED IMMEDIATELY, IT SHOULD BE ______ BUT NOT MORE THAN ____

REFRIGERATED; 24 HOURS

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95

PERFORMED TO RULE OUT UTI

URINE COLONY COUNT

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96

THIS IS ADDED TO MAINTAIN ACCURATE COLONY COUNT

BORIC ACID

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97

MANNER OF STREAKING FOR URINE COLONY COUNT

QUANTITATIVE

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98

DILTUON FACTOR FOR CFU/ML. 1 UL

1000

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99

DILTUON FACTOR FOR CFU/ML. 10 UL

100

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100

WHAT RESULT IS SIGNIFICANT FOR UTI

>100,000 CFU/ML

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