BACTE LAB MOCK EXAM

THIS IS A TYPE OF BSC THAT IS THE LEAST EFFECTIVE AS IT IS ENTIRELY OPEN

CLASS I

THIS IS A TYPE OF BSC THAT IS THE MOST USED IN LABORATORIES

CLASS II/LAMINAR FLOW

A SUBCLASS OF CLASS II BIOSAFETY CABINET THAT EXHAUST AIR INSIDE THE ROOM

IIA

A SUBCLASS OF CLASS II BIOSAFETY CABINET THAT EXHAUST AIR OUTSIDE THE ROOM

IIB

THIS IS THE MOST EFFECTIVE OF ALL CLASSES WHEREIN IT HAS A PART WHERE OBJECTS MAY PASS THROUGH

CLASS III

THIS BSC HAS BUILT IN GLOVES, SEALED TO THE CABINET

CLASS III

WHAT ARE BACTERIA/VIRUSES THAT ARE UNDER BSL-1

BACILLUS SUBTILIS AND MYCOBACTERIUM GORDONAE

WHAT ARE BACTERIA/VIRUSES THAT ARE UNDER BSL-2

SALMONELLA, SHIGELLA, STAPHYLOCOCCUS AUREUS, HIV, HBV

WHAT ARE BACTERIA/VIRUSES THAT ARE UNDER BSL-4

ARENAVIRUS, MARGBURG VIRUS

BSL-2 ORGANISMS ARE ACQUIRED THROUGH?

INGESTION, MUCUS MEMBRANE, AND PERCUTANEOUS EXPOSURE

WHAT ARE BACTERIA/VIRUSES THAT ARE UNDER THE CATEGORY A BIOTERRORISM?

SMALLPOX VIRUS, FRANCISCELLA, BACILLUS ANTHRACIS

WHAT ARE BACTERIA/VIRUSES THAT ARE UNDER THE CATEGORY B BIOTERRORISM?

RICKETTSIA, COXIELLA, BURKHOLDERIA PSEUDOMALLEI

WHAT ARE BACTERIA/VIRUSES THAT ARE UNDER THE CATEGORY C BIOTERRORISM?

YELLOW FEVER, VIRUS, DENGUE, EBOLA VIRUS, MERS-COV, ZIKA VIRUS

WHAT IS THE AGENT OF BLACK PLAGUE?

YERSINIA PESTIS

PRESENCE OF VIABLE BACTERIA IN THE BLOOD

BACTEREMIA

THIS IS A TYPE OF BACTEREMIA ACCOMPANIED BY SYSTEMIC INFLAMMATORY REPSONSE

SEPTICEMIA

WHAT ARE THE COMMON CONTAMINANTS IN BLOOD

S. EPIDERMIDIS, VIRIDANS STREPTOCOCCI, PROPIONIBACTERIUM ACNES, BACILLUS SPECIES, DIPHTHEROIDS

NEW NAME OF PROPIONIBACTERIUM ACNES?

CUTIBACTERIUM ACNES

ALL BACILLUS SPECIES ARE A COMMON BLOOD CONTAMINANT EXCEPT FOR?

BACILLUS ANTHRACIS

ANTICOAGULANT USED FOR BLOOD CULTURE

0.025% SPS

SPS IS AN INHIBITORY FOR?

NEISSERIA, PEPTOSTREPTOCOCCUS ANAEROBIUS, GARDNERELLA VAGINALIS, STREPTOBACILLUS MONILIFORMIS

REMEDY TO NEUTRALIZE THE EFFECT OF SPS

0.1% GELATIN

OTHER ADDITIVE USED FOR BLOOD CULTURE

SODIUM AMYLOSULFATE, 0.5-1.0% SODIUM CITRATE, HEPARIN

WHAT ADDITIVE IS USED TO RECOVER VIRUS IN THE BLOOD, CAN INHIBIT THE GROWTH OF SOME GRAM NEGATIVE BACTERIA AND NOT SUITABLE FOR THE RECOVERY OF FUNGI AS IT CAN INHIBIT YEAST

HEPARIN

THIS ADDITIVE IS NEVER USED IN BLOOD CULTURE

EDTA

MEDIA USED FOR BLOOD CULTURE

TRYPTICASE SOY AGAR, BRAIN HEART INFUSION, BRUCELLA BROTH

INDICATIONS FOR POSITIVE GROWTH IN THE BROTH INCLUDES PRESENCE OF

HEMOLYSIS, PELLICLE, TURBIDITY

BLOOD CULTURE IS SUBCULTURE IN THREE CULTURE MEDIA

CHOCOLATE AGAR PLATE, BLOOD AGAR PLATE, MACCONKEY AGAR PLATE

POSITIVE GROWTH WITHIN 1-3 DAYS IN SUB CULTURE MEDIA MEANS?

PATHOGENIC

POSITIVE GROWTH IN 5TH DAYS IN SUB CULTURE MEDIA MEANS?

CONTAMINANT

WHAT SPECIMEN IS USED TO DERECR TUBERCULOSIS AND PNEUMONIA

SPUTUM

WHAT BACTERIA IS PRESENT IN SPUTUM SPECIMEN?

MYCOBACTERIUM TUBERCULOSIS

WHAT BSL IS USED AS WELL AS BSC IN M. TUBERCULOSIS

BSL-3; BSC II

AGENTS OF PNEUMONIA

KLEBSIELLA PNEUMONIAE, STAPHYLOCOCCUS AURUES, PSEUDOMONAS AERUGINOSA

AGENTS FOR VENTILATOR-ASSOCIATED PNEUMONIA

STREPTOCOCCUS PNEUMONIAE, HAEMOPHILUS INFLUENZAE, STAPHYLOCOCCUS AUREUS, KLEBSIELLA ENTEROBACTER, SERRATIA

MEDIA USED FOR M. TUBERCULOSIS

LOWENSTEIN-JENSEN MEDIA AND MIDDLBROOKE 7H9 AND 7H10 BROTH

NO GROWTH IN LJ MEDIA AT 37 DEG WITHIN 8 WEEKS MEANS?

(-) MYCOBACTERIUM TUBERCULOSIS

(+) GROWTH IN LJ MEDIA AT 37 DEG WITHIN 2-3 WEEKS MEANS?

(+) MYCOBACTERIUM TUBERCULOSIS

WHAT IS THE APPEARANCE OF COLONIES IN M. TUBERCULOSIS HAVE IN LJ MEDIA

CAULIFLOWER LIKE

WHAT MEDIA IS USED TO RECOVER S. PNEUMONIAE

GENTAMICIN BAP

WHAT MEDIA IS USED TO RECOVER H. INFLUENZAE

BACITRACIN BAP

WHAT IS USED TO DETERMINE IF SAMPLE IS TRUE SPUTUM OR SALIVA

BARTLETT’S CLASSIFICATION

WHAT IS COUNTED IN BARTLETT’S CLASSIFICATION

POLYMORPHONUCLEAR NEUTROPHILS AND SQUAMOUS EPITHELIAL CELLS

ACCORDING TO BARTLETT’S CLASSIFICATION, WHAT IS TRUE SPUTUM?

PMN: >25 AND SEC: <10

ACCORDING TO BARTLETT’S CLASSIFICATION, WHAT IS SALIVA?

PMN: <25 AND SEC: >10

STORAGE TEMP FOR M. TUBERCULOSIS WHEN NO PROCESSED IMMEDIATELY

4 DEG CELSIUS (REFRIGERATOR TEMPERATURE)

HOW IS SPUTUM COLLECTED?

DEEP COUGH

RECOMMENDED VOLUME OF SPUTUM SPECIMEN FOR DIAGNOSIS OF M. TUBERCULOSIS

5-10 ML

GOLD STANDARD FOR DIGESTION AND DECONTAMINANT FOR MYCOBACTERIUM TUBERCULOSIS

N-ACETYL-L-CYSTEINE AND 1% SODIUM HYDROXIDE

LIQUEFY MUCOUS BY USING DIGESTANT/MUCOLYTIC AGENT

DIGESTION

THIS REMOVES CONTAMINANTS AND NORMAL FLORA

DECONTAMINATION

WHAT IS USED AS A DECONTAMINANT IF THE SPX IS LIKELY TO CONTAIN PSEUDOMONAS SPECIES

5-6% OXALIC ACID AND ZEPHIRAN-TRISODIUM PHOSPHATE

WHAT SPECIMEN IS GENERALLY USED FOR AEROBIC BACTERIA AND LEAST PREFERRED FOR ANAEROBIC BACTERIA

SWABS

MATERIALS FOR SWAB SPECIMEN

COTTON, DARCON, RAYON, CALCIUM ALGINATE

WHAT IS TOXIC TO NEISSERIA?

COTTON

THIS IS SAID TO BE TOXIC TO VIRUS

CALCIUM ALGINATE

THIS MATERIAL FOR SWAB IS SUITABLE FOR BACTERIA AND VIRUS

DACRON/RAYON

MAJOR THROAT PATHOGEN IS

STREPTOCOCCUS PYOGENES

S. PYOGENES CAUSES _____ WHICH OCCURS AMONG ___

PHARYNGITIS/STREPTHROAT; CHILDREN

WHICH BACTERIA IS KNOW TO HAVE A VIRULENCE FACTOR M PROTEIN

S. PYOGENES

A VIRULENCE FACTOR THAT IS RESPONSIBLE FOR THE SEQUALAE INFECTION OF S. PYOGENES

M PROTEIN

SEQUALAE INFECTION OF S. PYOGENES MAY LEAD TO?

RHEUMATOID HEART DISEASE AND ACUTE GLOMERULONEPHRITIS

A TEST TO DETECT ID THE CAUSE OF SORE THROAT IS S. PYOGENES

ANTISTREPTOLYSIN O TEST

WHAT IS THE MAJOR THROAT FLORA

VIRIDANS STREPTOCOCCI

IN IMMUNOCOMPROMISED PXS, V. STREPTOCOCCI MAY CAUSE _____

SUB-ACUTE BACTERIAL ENDOCARDITIS

BACTERIA FOR NASOPHARYNGEAL SWAB

BORDETELLA PERTUSSIS, NEISSERIA MENINGITIDIS, HAEMOPHILUS INFLUENZAE, MRSA, SARS-COV

CAUSATIVE AGENT OF WHOOPING COUGH

BORDETELLA PERTUSSIS

CAUSATIVE AGENT OF MENINGITIDIS

HAEMOPHILUS INFLUENZAE

THIS IS THE NORMAL FLORA OF ORO/NASOPHARYNX

NEISSERIA MENINGITIDIS

WHAT SPECIMEN IS USED FOR DIAGNOSING MENINGITIDIS

CSF

THIS CAN CAUSE MENINGITIDIS AMONG 5-29 YEARS OLD

NEISSERIA MENINGITIDIS

GENITAL SWAB IS USED FOR WHAT BACTERIA?

NEISSERIA GONORRHEAE

STORAGE TEMP OF N. GONORRHEAE

ROOM TEMPERATURE

WHICH SPECIMEN IS COLLECTED IF THE PATIENT IS INTUBATED OR IN A VENTILATOR

ENDOTRACHEAL ASPIRATE

THIS SAMPLE IS THE TOP PRIORITY IN THE BACTERIOLOGY LABORATORY

CEREBROSPINAL FLUID

MANNER OF COLLECTION OF CSF

LUMBAR TAP/SPINAL TAP

COLLECTION OF CSF (IN ORDER TUBES)

CHEMISTRY, MICROBIOLOGY, HEMATOLOGY

IN CASE ONLY ONE TUBE IS COLLECTED FOR CSF WHAT SHOULD BE THE ORDER OF DEPARTMENT

MICROBIOLOGY, HEMATOLOGY, CHEMISTRY

STORAGE TEMP OF CSF

ROOM TEMPERATURE

COMMON PATHOGENS IN CSF

CRYPTOCOCCUS NEOFORMANS, HAEMOPHILUS INFLUENZAE, NEISSERIA MENINGITIDIS, STREPTOCOCCUS AGALACTIAE, STREPTOCOCCUS PNEUMONIAE, LISTERIA MONOCYTOGENES

THIS CAN BE FOUND IN CSF BUT IS NOT CONSIDERED AS A BACTERIA BUT RATHER A CAPSULATED YEAST

CRYPTOCOCCUS NEOFORMANS

THIS IS USED TO VISUALIZE CRYTPCOCOCCUS NEOFORMANS

INDIA INK

MEDIA FOR DIAGNOSING PATHOGENS IN CSF

BAP, CAP

STOOL SPECIMEN IS USED FOR THE DETECTION OF WHAT PATHOGENS?

ENTERIC PATHOGENS

TRUE OR FALSE. URINE SPECIMEN IS INOCULATED STRAIGHT IN A PLATED MEDIA

FALSE

MEDIA FOR STOOL

SALMONELLA SHIGELLA AGAR, EOSIN METHYLENE BLUE, MCCONKEY AGAR

COMMON PATHOGENS FOUND IN STOOL

CAMPYLOBACTER JEJUNI, SALMONELLA, SHIGELLA, ESCHERICHIA COLI, VIBRIO SPECIES, YERSINIA ENTROCOLITICA

ALTERNATIVE SPECIMEN FOR STOOL

RECTAL SWAB

MOST COMMON CAUSE OF UTI

ESCHERICHIA COLI

COMMON CAUSE OF UTI AMONG SEXUALLY ACTIVE YOUN FEMALE

STAPHYLOCOCCUS SAPROPHYTICUS

PREFERRED MANNER OF COLLECTION IN URINE AND IS SUITABLE FOR THE RECOVERY OF AEROBIC BACTERIA

MIDSTREAM CLEAN CATCH

PREFERRED MANNER OF COLLECTION IN URINE FOR INFANTS AND IS SUITABLE FOR THE RECOVERY OF ANAEROBIC BACTERIA

SUPRAPUBIC ASPIRATION

URINE SHOULD BE PROCESSED WITHIN?

1 HOUR

IF URINE SPECIMEN IS NOT PROCESSED IMMEDIATELY, IT SHOULD BE ______ BUT NOT MORE THAN ____

REFRIGERATED; 24 HOURS

PERFORMED TO RULE OUT UTI

URINE COLONY COUNT

THIS IS ADDED TO MAINTAIN ACCURATE COLONY COUNT

BORIC ACID

MANNER OF STREAKING FOR URINE COLONY COUNT

QUANTITATIVE

DILTUON FACTOR FOR CFU/ML. 1 UL

1000

DILTUON FACTOR FOR CFU/ML. 10 UL

100

WHAT RESULT IS SIGNIFICANT FOR UTI

>100,000 CFU/ML