lec 14 slide 12

which reaction uses carbon fixation

dark reactions

central to carbon fixation is a reaction catalyzed by an enzyme called what

Rubisco

what does rubisco do

takes CO2 (which is INORGANIC) and converts it to an organic carbon form

when rubisco takes CO2 (which is INORGANIC) and converts it to an organic carbon form. what is it called

3-phosphoglycerate

what does rubisco stand for

ribulose bisphosphate carboxylase/oxygenase

what does rubiscos name ribulose bisphosphate carboxylase/oxygenase tell what what it does

carboxylate ribulose 1,5 bisphosphate (which is the substrate for Rubisco)

what does it mean for carboxylate a molecule?

COVALENTLY LINKING CO2

what is decarboxylation

removing CO2 SO carboxylation means adding CO2

what does the intermediate bounded to rubisco do

undergoes a hydrolytic cleavage here to make 2 molecules of 3-phosphogylcerate

in glycolysis, can , 3-phosphoglycerate is an glycolytic intermediate be used to make building blocks of the cell

YES

Is rubisco slow or fast enzyme

slow enzyme

how many molecules does rubisco turn per sec

3 molecules

what does it mean when rubisco only turns over 3 molecules per sec

so plants need a lot of this protein to survive

Rubisco is more than __% total protein in chloroplasts AND is the most abundant protein on earth

50

why is rubisco the most important/abundant protein on earth

because it is used by plants and microbes for photosynthesis

is the reaction catalyzed by Rubisco exergonic or endorgonic

exergonic

why is the reaction catalyzed by rubisco exergonic

ribulose 1,5-bisphosphate is a very high energy molecule so cell actually uses the NADPH and ATP from the light reactions to regenerate ribulose 1,5 bisP