Photosynthesis
conversion process in which light energy from the sun is captured by chloroplasts of plants and converted into chemical energy stored in sugar and other organic molecules
autotrophs
self- feeders. they sustain themselves without eating anything derived from other organisms.
photoautotrophs
organisms that use light to synthesize organic substances
heterotrophs
obtain organic material from compounds produced by other organisms
decomposers
consume the remains of dead organisms by decomposing and feeding on organic litter such as carcasses, feces, and fallen leaves
leaves are
major site of photosynthesis in plants
chlorophyll
the color of the leaf is from this. green pigment located within chloroplasts. absorbs light energy that drives the synthesis of organic molecules in the chloroplasts
mesophyll
chloroplasts are found mainly in these cells. it is the tissue that is on the interior of the leaf
stomata
co2 enters the leaf and oxygen exits by way of tehse microscopic pores
water absorbed by roots is delivered to leaves by
veins
stroma
envelope of two membranes encloses this dense fluid within the chloroplast
thylakoids
elaborate system of interconnected membranous sacs that segregate the stroma from another compartment, the interior of the thylakoids, or thylakoid space
grana
thylakoids are stacked in columns called this
chlorophyll resides in
thylakoid membranes
photosynthesis chemical equation:
6 co2 + 12 h20 + light energy > c6h1206 + 6 o2 + 6 h2o
the chloroplasts split
water into hydrogen and oxygen
a result of shuffling of atoms during photosynthesis is the
extraction of hydrogen from water adn its incorporation into sugar
O2
the waste product of photosynthesis in light reactions
reactants of photosynthesis
6 CO2 and 12 H2O
products of photosynthesis
C6H12O6 and 6 H2O and 6 O2
Light reactions
convert solar energy to chemical energy, light powers it, water is split in process, O2 is given off as byproduct, light reactions use light to reduce NADP+ to NADPH by adding a pair of electrons along with a H+
light reactions also
generate ATP using chemiosmosis to power teh addition of a phosphate group to ADP a process called photophosphorylation
light energy is initally converted into
NADPh and ATP
light reactions produces no
sugars. this happens in the calvin cycle
calvin cycle begins by
incorporating CO2 from the air into organic moelcuels already present in the chloroplast
site of light reactions
thylakoids of the chloroplast
calvin cycle occurs in
the stroma
Chloroplast's thylakoids transform
light energy into chemical energy of ATP and NADPH
wavelength
distance between crests of electromagnetic waves
electromagnetic spectrum
the entire range of radiation
segment of light spectrum most important to us is
narrow band between 380 and 750 nm because it is visible light and detectable by human eye
photon
light behaves as though it consists of discrete particles called these, they have a fixed quanitty of energy
the shorter the wavelength of light..
the greater the energy of each photon of that light
pigments
substances that absorb visible light
wavelengths that are absorbed
disappear
spectrophotometer
this machine directs beams of light of different wavelengths through a solution of the pigment and measures the fraction of the light transmitted at each wavelength
absorption spectrum
a graph plotting a pigment's light absorption versus wavelength
chlorophyll a
its absorption spectrum suggests that violet blue adn red light work best for photosynthesis since they are absorbed, while green light is the least effective
action spectrum
profiles the relative effectiveness of different wavelengths of radiation in driving the process.
an action spectrum is prepared by
illuminating chloroplasts with light of different colors and then plotting wavelengths against some measuer of photosynthetic rate, such as co2 consumption or o2 release.
chlorophyll b-
one of the accessory pigments that is another form of chlorophyll. it is almost identical to chlorophyll a but a slight structural difference between them is enough to give the two pigments slightly different absorption spectra
chlorophyll a colors
blue- green
chlorophyll b colors
yellow- green
carotenoids
accessory pigments that are hydrocarbons that are various shades of yellow and orange because they absorb violet and blue- green light
photoprotection
most important function of carotenoids. they absorb and dissipate excessive light energy that would otherwise damage chlorophyll or interact with oxygen, forming reactive oxidative molecules that are dangerous to the cell
what exactly happens when chlorphyll and other pigments absorb light?
the colors corresponding to teh absorbed wavelengths disappear from the spectrum of the transmitted and reflected light, but energy cannot disappear.
photosystem
composed of a reaction center surrounded by a number of light-harvesting complexes
light-harvesting complex
consists of pigment molecules (which may include chlorophyll a, b, and carotenoids) bound to particular proteins
the number and variety of proteins allows a photosystem to
harvest light over a larger surface adn a larger portion of the electromagnetic spectrum than any single protein could alone do
light- harvesting complexes act as an antenna
when a pigment molecule absorbs a photon, the energy is transferred from pigment molecule to pigment molecule within a light- harvesting complex until it is funneled into the reaction center
reaction center
protein complex that includes two special chlorophyll a molecules and a molecule called the primary electron acceptor
the chlorophyll a molecules in the primary electron acceptor are special because
their molecular environment- their location and other molecules with which they are associated- enables them to use the energy from light to boost one of their electrons to a higher energy level
first step in light reactions
solar powered transfer of an electron from a special chlorophyll a molecule to the primary electron acceptor
as soon as the chlorophyll a molecule reaches a higher energy level
the primary electron acceptor captures it
the thylakoid membrane is populated by
two types of photosystems that cooperate in the light reactions of photosynthesis. They are photosystem I and photosystem II. Photosystem II functions first
each photosystem has a
reaction center with a particular kind of primary electron accpetor next to a pair of special chlorophyll a molecules associated with specific proteins
reaction center chlorophyll a of photosystem II
known as p680 because this pigment is best at absorbing light having a wavelength of p680 (red wavelength)
reaction center chlorophyll a of photosystem I
p700 because this pigment is best at absorbing light having a wavelength of p700
the two pigments p700 and p680 are
identical in chlorophyll a molecules. their association with different proteins in the thylakoid membrane affects the electron distribution in teh chlorophyll molecuels and accounts for the slight difference in teh light absorbing proteins
what drives the synthesis of NADPH and ATP by energizing the two photosystems embedded in the thylakoid membrane?
light!
what is the predominant route in light reactions?
noncyclic electron flow
calvin cycle is
anabolic, building sugar from smaller molecules and consuming energy
carbon enters the calvin cycle in the form of
co2 and leaves in the form of sugar. the cycle spends ATP as an energy source and consumes NADPH as reducing power for adding high energy electrons to make the sugar
for every three molecules of co2 synthesized
one molecule of G3P is produced
phase one of the calvin cycle:
carbon fixation- incorporates each CO2 molecule by attachign it to a five carbon sugar named RuBP. the enzyme that catalyzes the raction is Rubisco. product of this reaction is a six carbon intermediate that immediately splits in half to form two molecules of 3- phosphoglycerate
phase 2 of the calvin cycle
reduction- each molecule of 3- phosphoglycerate receives an additional phosophate group from ATP,. next a pair of electrons that originated from NADPH reduces it to G3P.
phase 3 of the calvin cycle
regeneration of the co2 acceptor RuBP- carbon skeletons of five molecules of G3p are rearranged into three molecules of RuBP. the cycle spends three more molecules of G3P.
inputs into the calvin cycle
9 atp 6 nadph 3 co2
outputs
1 g3p 9 adp 6 nadp+