Lab Procedures Set 7

5.0(1)
studied byStudied by 1 person
learnLearn
examPractice Test
spaced repetitionSpaced Repetition
heart puzzleMatch
flashcardsFlashcards
Card Sorting

1/30

flashcard set

Earn XP

Description and Tags

Study Analytics
Name
Mastery
Learn
Test
Matching
Spaced

No study sessions yet.

31 Terms

1
New cards
Microhematocrit
A measurement of the relative RBC volume for a (small quantity) volume of whole blood spun down in a capillary tube
2
New cards
Microhematocrit Procedure
The capillary tube with whole blood is spun down in a centrifuge, RBCs pack at the bottom, hematocrit is the percent of the tube filled with RBCs.  
3
New cards
What makes up the buffy coat? Is it included in the hematocrit measurement?
WBCs and platelets make up the buffy coat and are not included in the hematocrit measurement.  
4
New cards
Why is the rule of three important?
 

The result has to agree with the rule of three. If it doesn’t, the hemoglobin or hematocrit would be inaccurate.  
5
New cards
Difference between microhematocrit and hematocrit?
*Microhematocrit is considered the manual version of measuring hematocrit vs hematocrit on the analyzer* 
6
New cards
Why is the microhematocrit higher than the automatic hematocrit?
 

Microhematocrit will be a little bit higher than hematocrit because oof the plasma trapped in erythrocytes.  
7
New cards
Erythrocyte Sedimentation Rate
Nonspecific screening test indicating inflammation based on how quickly RBCs stack and settle
8
New cards
Increased ESR associated diseases
Kidney disease, pregnancy, rheumatic fever, rheumatoid arthritis, anemia, syphilis, lupus, thyroid disease, elevated room temperature
9
New cards
Decreased ESR associated diseases
Congestive heart failure, hyper viscosity, decreased fibrinogen, polycythemia, sickle cell anemia 
10
New cards
Westergren method
uses Na citrate diluent with whole blood, read at 60 minutes 
11
New cards
Automated ESR method
on the Alcor, detects rouleaux via flow cell and photometry 
12
New cards
Manual reticulocyte count
finds the bone marrow RBC production index, most effective measure of erythropoiesis, counted out of 1000 RBCs
13
New cards
Decreased Retic associated diseases
Aplastic anemia, exposure to radiation, chronic infection, medications, megaloblastic anemia 
14
New cards
Increased Retic associated diseases
rapid blood loss, high elevation, hemolytic anemia, medications, pregnancy 
15
New cards
Miller eye disc
helps to not count 1000 RBCs, provides standardized area 
16
New cards
How to perform a manual retic
*Mix equal parts stain and whole blood for the manual retic, count all reticulocytes in the large and small square, count small squares until you get to 112 RBCs* 
17
New cards
Manual retic formula
Retic % = Retics in large square / (RBCs in small square \* 9) \* 100 % 
18
New cards
What stain is used for the reticulocyte counts?
 

New Methylene Blue  
19
New cards
Microhematocrit: sources of error
not filling the capillary tube, reading the buffy coat, not following the rule of three 
20
New cards
ESR: sources of error
not keeping the tube free from vibration, not keeping the tube level, not pushing the blood to the zero mark, not replacing the cap 
21
New cards
Manual retic: sources of error
Interferences: nucleated erythrocytes, Howell-jolly bodies, Pappenheimer bodies, cold agglutinins, basophilic stippling, heinz bodies, parasites, autofluorescence 
22
New cards
Percent retic, RPI formula
Retic % = Retics in large square / (RBCs in small square \* 9) \* 100 % 
23
New cards
Conditions associated with increased ESR and retic counts
* Pregnancy  
* Hemolytic anemia  
24
New cards
Conditions associated with decreased ESR and retic counts
Aplastic anemia / sickle cell anemia  
25
New cards
Soucres of error on the hemocytometer
 

Not evenly distributing the object across the hemocytometer.  

\
Overcrowding the hemocytometer.  
26
New cards
Cytospin
concentrates the cells into a smaller area of the slide
27
New cards
Principle of the LAP test
**Helps differentiate CML from a leukemoid reaction**

\
Naphthol AS-BI is hydrolyzed by the enzyme at an alkaline pH. The hydrolyzed substrate with a red-violet LB or fast blue BB dye produces a colored precipitate at the site of enzyme activity
28
New cards
Decreased LAP =
Untreated CML
29
New cards
During a __**Leukemoid reaction**__ LAP changes from…
High normal to increased
30
New cards
High LAP =
Pregnancy, polycythemia vera 
31
New cards
Low LAP =
PNH, Sideroblastic Anemia, Myelodysplastic disorders