ch 14 dna structure & function

fredrick griffith

reported first demonstration of bacterial transformation

nucleotides

• building blocks

• 5-carbon sugar, phosphate, & nitrogenous base

pyrimidine

single six carbon ring

cytosine & thymine

purine

six carbon + five carbon ring

adenine & guanine

what is its sugar?

deoxyribose

how is dna read?

5’ to 3’

what direction is DNA “woven” together ?

antiparallel

bond between nucleotides (bases)?

hydrogen bond

bond between sugar and phosphate?

phosphodiester bond

dideoxy chain termination (dct)

• one of the sequencing technique

• chain terminator: dideoxynucleotides (ddNTPs)

steps for sequencing (dct)

1. DNA is denatured