lactate dehydrogenase

• Interconversion of lactic acid and pyruvic acid

lactate dehydrogenase

coenzyme used by ldh

nadh

high activities tissue sources of lactate dehydrogenase

heart liver kidney skeletal muscle erythrocyte

molecular weight of lactate dehydrogenase

128 kda

how many subunits of lactate dehydrogenase

4

desginated subunits of lactate dehydrogenase

h and m

migrates fastest toward the anode

LDH-1:

migrate the slowest to anode

ldh 5

in sera of healthy individuals what isoenzyme has the highest fraction

ldh 2

aside from ldh 2 rank the order of highest percentage

1 3 4 5

has higher affinity for lactate

ldh 1 ldh 2

has higher affinity for pyruvate

ldh 4 ldh 5

normal rang of ldh

125 to 220 u/l

elevated ldh indicates

non specific finding

diseases with highest levels of ldh as symptoms

pernicious anemia hemolytic disorder

if ldh 1 is higher than ldh 2 in serum what does it indicate

ldh flip patter

in acute myocardial infraction ldh rises

12-24 hours

in acute myocardial infraction ldh peaks

48-72 hours

in acute myocardial infraction ldh returns to normal after

10 days

in cases where total LDH is increased, and the relative amount of each isoenzyme is roughly the same what does it show

tombstone pattern

tombstone pattern is seen on persons with

shock and hypoxemia

what is ldh 6 (death)

alcohol dehydrogenase

sources of error when measuring lactate dehydrogenase

hemolysis exercise