MOCK QUIZ (BACTE LAB)

In a catalase test, it produces a bubbling formation/effervescence in what bacteria?

a. Staphylococcus

b. Streptococcus

c. Enterococcus

d. Micrococcus

Select all that applies. LETTER ONLY

A and D

MSA is a selective media for what bacteria?

a. Micrococcus

b. Streptococcus

c. Neisseria

d. Staphylococcus

e. None of the choice

f. A and D

g. A and B

h. B and D

F

These are gram positive cocci, oxidase negative and are positive in DNAse test

Staphylococcus aureus

What is/are the species that has an enzyme Beta-lactamase?

Staphylococcus aureus

Voges Proskauer Test is a differential test for what bacteria?

a. S. hyicus

b. S. epidermidis

c. S. aureus

d. S. pneumonia

e. S. agalactiae

f. B and C

g. A and C

h. D and E

i. None of these

i

A gram positive cocci, glucose fermenters, resistant to bacitracin shows a red color in Voges-Proskauer Test

a. S. aureus

b. S. intermedius

c. S. epidermidis

d. S. pneumoniae

A

If the catalase test is positive and the coagulase test shows negative, what would be the next step?

a. Report as negative

b. Oxidase test

c. Bacitracin susceptibility

d. Novobiocin Susceptibility

e. Repeat test

f. PYR test

g. CAMP test

B and C

Which of the following bacteria are susceptible to Novobiocin?

a. S. epidermidis

b. S. aureus

c. S. saprophyticus

d. S. lugdunensis

e. S. haemolyticus

f. S. intermedius

g. All of the above

h. None of the choices

A, C, D, E

Which of the bacteria are coagulase, oxidative, gelatinase, DNAse negative

a. S. aureus

b. S. epidermidis

c. S. saprophyticus

d. S. pneumoniae

e. C and D

f. B and A

g. B and C

G

PYR Test for Beta-hemolytic catalase negative is positive in what bacteria?

a. S. agalactiae

b. S. pyogenes

c. E. faecalis

d. E. durans

e. All of the above

f. None of the choices

B

Bacitracin Susceptibility Test in catalase negative is susceptible in which bacteria?

a. S. agalactiae

b. S. pyogenes

c. E. faecalis

d. E. durans

e. All of the above

f. None of the choices

B

Bacitracin Susceptibility Test in catalase negative is resistant in which bacteria?

a. S. agalactiae

b. S. pyogenes

c. E. faecalis

d. E. durans

e. All of the above

f. None of the choices

A, C, D

SXT Susceptibility Test in catalase negative is resistant in which bacteria?

a. S. agalactiae

b. S. pyogenes

c. E. faecalis

d. E. durans

e. All of the above

f. None of the choices

A and B

SXT Susceptibility Test in catalase negative is susceptible in which bacteria?

a. S. agalactiae

b. S. pyogenes

c. E. faecalis

d. E. durans

e. All of the above

f. None of the choices

F

Camp Test is a presumptive test for?

a. Group B streptococci

b. Group A streptococci

c. Streptococcus agalactiae

d. Streptococcus pyogenes

e. A and C

f. B and D

g. A and D

h. B and C

E

Hippurate Hydrolysis Test is a differential test for which bacteria?

a. Group B streptococci

b. Group A streptococci

c. Streptococcus agalactiae

d. Streptococcus pyogenes

e. A and C

f. B and D

g. A and D

h. B and C

E

Positive reaction of Hippurate Hydrolysis Test shows what color?

a. Reddish pink

b. Deep purple

c. Deep blue

d. Turbid

B

Which of the following is the reagent/substrate of PYR test:

a. P-dimethylaminocinnamaldehyde

b. L-pyrrolidone and B-naphthylamide

c. L-pyrrolidonyl-B-naphthalamide

d. L-pyrrolidonyl-arylamidase/L-pyrrolidonyl-aminopeptidase

e. None of the choices

C

Which of the following is the inhibitor of PYR test:

a. P-dimethylaminocinnamaldehyde

b. L-pyrrolidone and B-naphthylamide

c. L-pyrrolidonyl-B-naphthalamide

d. L-pyrrolidonyl-arylamidase/L-pyrrolidonyl-aminopeptidase

e. None of the choices

E

What color does PYR test produces when it tests positive

a. Red

b. Pink

c. Blue

d. Deep purple

A

What test/s is/are considered to be a presumptive test for S. pyogenes?

a. Bacitracin Susceptibility Test

b. Camp Test

c. Hippurate Hydrolysis Test

d. Sulfamethoxazole Trimethoprim Susceptibility Test

e. None of the choices

A and D

If negative in PYR test and resistant to Bacitracin, perform the following:

a. SXT test

b. Bacitracin Susceptibility Test

c. CAMP test

d. Hippurate Hydrolysis Test

e. None of the choices

C and D

What agar is used in the CAMP test?

a. MSA

b. CAP

c. BAP

d. B and C

e. A and C

C

Incubation for CAMP test should be at _______ for _____ ?

a. 35-37C for 24 hours

b. 35-37C for 2 hours

c. 35-37C for 10-15 minutes

d. None of the choices

A

Incubation for Hippurate Hydrolysis test should be at _______ for _____ ?

a. 35-37C for 24 hours

b. 35-37C for 2 hours

c. 35-37C for 10-15 minutes

d. None of the choices

B

What test uses a reagent of B-lysin Disk

a. Hippurate Hydrolysis test

b. CAMP test

c. SXT test

d. PYR test

e. Bacitracin Susceptibility Test

B

CAMP test identifies the CAMP factor that is present in which bacteria?

a. S. agalactiae

b. S. pyogenes

c. S. aureus

d. S. pneumonia

e. None of the choices

A

Optochin Susceptibility Test/Taxo P helps in differentiating?

a. Alpha-hemolytic streptococci

b. Beta-hemolytic streptococci

c. Gamma-Hemolytic Streptococci

d. All of the above

A

Optochin Susceptibility Test/Taxo P is susceptible in what bacteria?

a. S. pyogenes

b. S. pneumoniae

c. Viridans streptococci

d. Non-enterococci

e. Enterococci

f. D and E

B

Which test has a clearing of turbidity in Positive reaction?

a. Bile Esculin Test

b. Optochin Susceptibility Test

c. Bile Solubility Test

d. Salt tolerance Test

C

Incubation for Bile Solubility test should be at _______ for _____ ?

a. 35-37C for 24 hours

b. 35-37C for 2 hours

c. 35-37C for 10-15 minutes

d. None of the choices

C

A presumptive test for the identification of Group D streptococci

a. Bile Solubility Test

b. Bile Esculin Test

c. Optochin Susceptibility test

d. Salt tolerance test

e. None of the choices

B

What is the indicator for Bile Esculin Test?

a. Phenol Red

b. DMACA

c. Ferric Ammonium Citrate

d. there is no indicator in Bile Esculin Test

C

Blackening of media in Bile Esculin Test is true in which bacteria?

a. Group D

b. Shigella

c. Enterococcus

d. Non-enterococcus

e. A and B

f. C and D

A, C, D

Negative reaction in Bile Esculin Test is true of which bacteria?

a. Enterococcus

b. Non-enterococcus

c. E. faecium

d. Viridans Streptococcus

e. All of the above

f. None of the choices

D

A test that is differential for Enterococci from other Group D Streptococci

a. Bile Solubility Test

b. Bile Esculin Test

c. Optochin Susceptibility test

d. Salt tolerance test

e. None of the choices

D

Which of the following test has a media of Nutrient broth with 6.5% Sodium chloride

a. Bile Solubility Test

b. Bile Esculin Test

c. Optochin Susceptibility test

d. Salt tolerance test

e. None of the choices

D

Salt tolerance test shows a Positive growth in ___________ and no growth in_________

a. Enterococcus; non-enterococcus

b. Non-enterococcus; enterococcus

c. S. pneumoniae; Viridans streptococci

d. Viridans streptococci; S. pneumoniae

A

The pigment production of S. aureus is best at what temperature?

a. Room temperature

b. 20-25C

c. 37C

d. Hot temperature

e. None of the choices

A and B

The optimal temperature for bacterial growth of S. aureus is?

a. Room temperature

b. 20-25C

c. 37C

d. Hot temperature

e. None of the choices

C

Gram positive cocci seen in cluster, modified oxidase negative and catalase positive that has a colony characteristics of gray-white, slime-producing and non-hemolytic in Blood Agar Plate

a. S. epidermidis

b. S. saprophyticus

c. S. lugdunensis

d. S. haemolyticus

e. All of the above

A

Gram positive cocci seen in cluster, modified oxidase negative and catalase positive that has a colony characteristics of opaque, glossy, usually white in Blood Agar Plate

a. S. epidermidis

b. S. saprophyticus

c. S. lugdunensis

d. S. haemolyticus

e. All of the above

B

Gram positive cocci seen in cluster, modified oxidase negative and catalase positive that has a colony characteristic unpigmented in Blood Agar Plate

a. S. epidermidis

b. S. saprophyticus

c. S. lugdunensis

d. S. haemolyticus

e. All of the above

C

Gram positive cocci seen in cluster, modified oxidase negative and catalase positive that has a colony characteristics of opaque and non-hemolytic in Blood Agar Plate

a. S. epidermidis

b. S. saprophyticus

c. S. lugdunensis

d. S. haemolyticus

e. All of the above

D

Which test has a reagent of 3% Hydrogen Peroxide

Catalase

What is the reason why we can have a false positive in a catalase test

Pseudoperoxidase

What is the reagent used in coagulase test?

Rabbit’s plasma

Screening of Coagulase test

Slide Coagulase Test

Confirmatory test in Coagulase Test

Tube Coagulase Test

Which bacteria shows a negative reaction in Coagulase test?

CoNS and Micrococcus

In coagulase test, Incubate for _____ at ____.

1-4 hours; 37C

MSA is the selective media for which bacteria?

Staphylococcus and Micrococcus

MSA is the differential media for which bacteria?

S. aureus from other Staphylococcus species

Color of MSA

a. Reddish pink

b. Deep purple

c. Deep blue

d. Turbid

A

pH indicator of MSA

Phenol red

Coagulase positive that are halophilic and mannitol fermenters

Staphylococcus aureus

These are catalase positive that are halophilic and are not mannitol fermenters

CoNS and Micrococcus

Two indicators in DNAse

Methyl green and Toluidine blue

Which indicator in DNAse causes lysis of DNA

Methyl green

Reagent for Nitrate Reduction Test

Reagent A (Sulfanilic acid), Reagent B (Alpha-naphthylamide), Zinc dust/powder

Color of Nitrate Reduction Test when tested positive?

Red

Incubation of Nitrate Reduction Test

24 hours at 37C

Incubation of Voges-Proskauer Test

24-48 hours at 37C

During the incubation Acetoin test, if the bacteria utilize glucose in the Butylene Glycol Pathway it will degrade glucose into?

Acetoin or Acetyl Methyl Carbinol

Gelatinase liquefaction is a test for which bacteria?

Staphylococcus aureus

What are the bacteria positive in PYR test

S. aureus and S. pyogenesis

Indicator for PYR test

P-dimethylaminocinammaldehyde

Color of PYR test when tested positive?

Pink or red

In Modified Oxidase Test, Oxidase reagent will react to ______ in the bacteria producting what color

Cytochrome C; blue color (indophenol)

WHat bacteria is negative in Modified oxidase test

CoNS and Staphylococcus aureus

Incubation of Modified Oxidase Test

Room temperature for 2 minutes

WHAT IS THE INDICATOR OF HUGH AND LEIFSON

Bromthymol Blue

WHAT IS THE INDICATOR OF CDC METHOD

PHENOL RED

WHAT TESTS CAN BE A DIFFERENTIAL TEST FOR YOUR CONS AND MICROCOCCUS

BACITRACIN SUSCEPTIBILITY AND NOVOBIOCIN SUSCEPTIBILITY

iNOCULATION USED FOR NOVO AND BACITRACIN TEST

OVERLAP STREAK METHOD

INCUBATION FOR NOVOBIOCIN TEST

24 HOURS AT 37C

INCUBATION FOR BACITRACIN TEST

24 HOURS AT 87C

DETECTS INDUCIBLE RESISTANCE OF SOME S. AUREUS STRAINS AGAINST CLINDAMYCIN

D-ZONE TEST

INDUCIBLE CLINDAMYCIN RESISTANCE SHOWS A?

D-SHAPED ZONE OF INHIBITION

Catalase negative that are gram positive in pairs

Streptococcus pneumoniae

Gram positive in chains, catalase negative, and are obligate aerobes?

Peptostreptococcus