Blood plasma and plasma proteins

What clotting factors are removed to make serum.

2,5,8

In serum formation blood is allowed to clot and then clot is retracted (TIF)

True

What leads to clot having high serotonin content.

Breakdown of platelets during clotting

Plasma protein concentration

6.4 to 8.3 g/ DL

What is the original classification of protein plasma. By whom was it done?

Howe (1922) it was based on precipitation of salts

What are the fractions of plasma proteins

I. Albumin 2. Globulin (alpha 1, alpha2, beta, gamma 3 fibrinogen

Electrophoretic protein patterns (only to see)

Basis of paper electrophoresis proteins are identified

Molecular weight I shape of plasma protein

Fibrinogen has highest molecule or weight (500,000), the least is of albumin (69,000)

The plasma exert a ________ pressure of ____ mm Hg.

,

Oncotic, 25

What is isoelecthic point?

The ph value of the protein when it has achieved electric neutrality ie : positive and negative ion is same.

What is electrophoretic mobility?

The ability of a palein to act as an anion in alkaline solution and cation in acidic solution.

What method ultilises the property of proteins to be precipitated by various salts.

Method of separation of proteins

What solution is used to precipitate plasma protein fractions

Ammonium sulphate

Which protein is precipitated in full saturation

Albumin

Globulin is precipitated in what concentration? Condo are the further sections of globulin.

Half saturation. Englobin → 1/3 sat, rest → pseudogloubin

Fibrinogen precipitation is in what sat?

1/5

What makes protein molecules soluble in water?

Polar residues → NH 2 and COOH

What is the nature of proteins? ( acidic / amphoteric/ alkaline) and why.

Amphotericpresence of NH 2 and COOH

What mall plasma proteins excellent buffers?

Their amphoteric nature

Features of albumin. (4)

1. Abumin→69000, prealbumin→60,000 2. Plasma levels→ 48g/ DL 3. Synthesis occurs in liver 4 halflife is 10 days.

Globulin features (3)

Plasma levels → 2.3g/dl

Molecular weight → 90,000 to 150,000

Types → Alpha 1, alpha 2, beta 1, beta 2, gamma

Glycoproteins consist of?

Carbohydrate and proteins

Lipoproteins consist of?

Globulin and lipid

types of lipoprotein

High density, low density, very low density, chylomicron, transferrin, haptoglobin, ceruloplasmin, fetuin, immunoglobin, angiotensinogen, Haemagglutinins.

What are high density lipoproteins

Alpha lipoprotein, 50% protein and large amount of fat and cholesterol

What ore low density lipoproteins

Beta, large amountf glycosides

What are very low density lipoproteins

Beta, high amounts of triglycerides and cholesterol

What ore ihylomicions

98. % Triglycerides

Transferrin

90,000 molecular weight, alpha 2 beta protein, iron binding property useful for transport and storage, binds to two atoms of ferric. iron

Cerutoplasmin

Binds with copper, transport and storage

Ferris

Growth promoting protein in newborns

Haemagglutinins

Took against RBC antigen

Fibrinogen features

0.3 g / DL , 500,000, six nogreptide chain ( alpha 2, beta 2 gamma 2) joined by disulphide bonds., clotting factor

Prothrombin

40 mg / DL, 68,000, liven synthesis promoted by vitamin k