Guest Lecture Week 10

Spatial resolution & temporal resolution quality for lesions

Good spatial

Poor temporal (permanent damage, can’t contrast)

Spatial resolution & temporal resolution quality systemic injections of drugs

Very poor spatial —> don’t know where its targetting

Good temporal resolution —> temporary

Spatial resolution & temporal resolution quality for intercranial injection of drugs

Good spatial —> targeting specific brain region

• Still pretty poor, since in vivo

Good temporal

Spatial resolution & temporal resolution quality for electrophysiological recordings

Good spatial & temporal

• Milliseconds of change detect + control

How do electrophyisological recordings work & cons?

They use electrodes to control and detect millisecond changes in the brain

Data is strictly observational

Spatial resolution & temporal resolution quality for optogenetics

Very good temporal + spatial

• Specific & shows strong causality

Spatial resolution & temporal resolution quality for chemogenetics

Good spatial

Okay temporal

Infralimbic cortex findings with extinction + drug used

IL needed for extinction consolidation via muscimol

Infralimbic cortex findings with enhancing extinction learning + drug used

Using PTX (Gaba antagonist) to stimulate IL in extinction consolidation

No effect acquisition of extinction

What evidence did optogenetics provide for enhancing extinction learning

Found silencing via opsins in 2nd extinction also reduced freezing