Biotech chapter 4 mc questions

How is gene expression controlled in prokaryotic cells?

operons

How often will cells divide under ideal growing conditions?

every 20 minutes

What is a possible outcome of site specific mutagenesis?

all of the above

Which of the following is an example of sterile technique?

treating a lab bench with disinfectant

All of the following are reasons to use TRIS during electrophoresis except

to stain the molecules

A circular plasmid was cut with the restrictive enzyme and three bands where visualized after electrophoresis. How many cuts were made?

three

which agarose concentration would work best for separating very large molecules?

0.8

What is agar?

the solid medium upon which bacteria is grown

Semi conservative replication means...

each new DNA molecule has half of the old one

The sequence of nitrogenous bases on one strand of DNA may determine the sequence of

amino acids in a protein molecule

Nucleotides are connected together in a DNA molecule by

phosphodiester bonds

How many hydrogen bonds hold base pairs together?

two for A & T

three for G & C

All of the following regulate gene expression in eukaryotic cells except

operators

Prokaryotic DNA is contained in

the cytoplasm

In eukaryotic cells after an mRNA has been transcribed

introns are removed

Eukaryotic gene expression can be increased by

enhancers

Most of the DNA in humans is

spacer DNA within and between the genes

The anti-parallel nature of double stranded DNA means

both a and b are correct

The order of amino acids on a polypeptide chain is encoded by

mRNA

Which stain causes DNA to glow orange when exposed to UV light?

ethidium bromide

When a regulatory operator binds to a molecule

gene expression is turned off

Genes code for

all of the above

A replication fork is

an area where DNA separates in two

How is the packaging of DNA different in eukaryotes and prokaryotes?

Eukaryotes have more cells and they are linear

All of the following are reasons some people need recombinant protein therapy except

strong hair and nails

How do DNA molecules vary from one species to another?

the number of genes and non coding areas

All of the following may be used to induce changes at a particular locus on a DNA molecule except

low temperatures

A typical E. coli genome contains about _____ genes

2000

R plasmids are useful for both bacteria and scientists because they both contain

one or more antibiotic resistant genes

A linear piece of dna was cut with a restriction enzyme and four bands where visualized after electrophoresis

3