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Key Concepts: Cell Theory, Cell Structure & Function, Prokaryotic vs. Eukaryotic, Cell Membrane, Microscopes, Passive vs. Active Transport, Diffusion, Osmosis, Energy (Potential vs. Kinetic), Photosynthesis & Cell Respiration (Aerobic vs. Anaerobic), Fermentation (Lactic Acid & Alcoholic) 1. Describe one similarity and one difference between the two terms in each of the following pairs: a. Eukaryote, prokaryote Eukaryote: Has a membrane-bound nucleus in the cell Prokaryote: No nucleus, DNA free-floating in the cell, can have flagellum Both: have cell membranes, have DNA, have ribosomes b. Cell wall, cell membrane Cell wall: rigid, not as flexible, more selective (harder for things to pass through) Cell membrane: fluid, flexible, selectively permeable Both: enclose cell, facilitate what goes in/out of cell c. Diffusion, facilitated diffusion Diffusion: movement of particles from high to low concentration Facilitated diffusion: movement of particles through channel proteins Both: are passive transport (no energy required), particles move from HIGH to LOW 2. Describe the structure of a phospholipid bilayer. There are 2 layers of phospholipids (consisting of hydrophilic heads and hydrophobic tails) 3. Explain the following diagram using the terms: diffusion, cell membrane, low concentration, energy, high concentration. The water molecules are moving across the cell membrane to reach a state of equilibrium. The molecules move from HIGH to LOW concentration, so they move downwards across the membrane. This is an example of diffusion, or passive transport - this does not require energy because it is fueled by the difference in concentrations. 4a. What is the microscope magnification of the eye piece? scanning? low power? high power? ● Eye piece = 10x ● Scanning = 4x ● Low power = 10x ● High power = 40x b. If you were looking at an onion cell using the high power lens, what is the TOTAL MAGNIFICATION at which you are looking at the cell? High power = 40x Eye piece = 10x 40 x 10 = 400x Your total magnification would be 400x using the higher power objective lens. 5. Identify the difference between hypertonic, isotonic, and hypotonic solutions: A = isotonic B = hypotonic C = hypertonic 6. How is active transport different from diffusion and facilitated diffusion? ● Active Transport = requires energy, molecules are forced AGAINST the gradient from LOW to HIGH concentration ● Diffusion = does not require energy, molecules move from HIGH to low concentration ● Facilitated diffusion = does not require energy, molecules move from HIGH to low concentration, but it requires the help of channel proteins (typically larger molecules) 7. Explain what is happening in the following picture. This is an example of ENDOCYTOSIS (Active Transport) - there is a chemical/nutrients being taken into the cell when it’s engulfed by the cell membrane (becomes a vesicle). 8. What is the difference between potential and kinetic energy? Give an example of each. a. Poyential energy - stored energy; e.g,glucose, a ball at the topof ahill b. Kinetic energy - energy of motion e.g., a car onthe freeway 9. Write out the full chemical reaction for PHOTOSYNTHESIS. What organelle is responsible for this? Chloroplast 10. Write out the full chemical reaction for CELL RESPIRATION. What organelle is responsible for this? Mitochondria 11. If we are at 400X magnification (field diameter is 450 micrometers), and there are 10 cells that fit across the diameter of what we’re seeing, what is the estimated size of ONE cell? 450 nanometers / 10 cells = 45 nanometers per cell 12. What is the role of the stomata? What is the role of the guard cells? Stomata role isgas exchange to let oxygen and carbondioxide pass through, as needed for key processes such as photosynthesis and cellular respiration. Guard cells arepairs of cells that surround the stomata and controlgas exchange by regulatingthe openingand closure of stomata. 13. What would cause guard cells to swell and open stomata? What would cause guard cells to shrink and close stomata? → Whentheplanthas anexcess of water, theguard cells swell and create anopeningfor the exchange ofgas → Whentheplanthas a lack of water, theguard cells shrink and close the openingfor the exchange ofgas 14. In fermentation, what relationship exists between the amount of available sugar and amount of carbon dioxide produced? → As more sugar is available, there will be more fermentationthathappens, and more carbondioxideproduced → There is a direct relationshipbetweenthose two factors 15. List the # of ATP produced by each of the following: Glycolysis = 2 ATP Krebs = 2 ATP ElectronTransport = 34 ATP 16. Explain one example of lactic acid fermentation. Explain one example of alcoholic fermentation. a. Lactic acid fermentation- heating milk and combiningit withtwo live bacteria cultures, resultinginthe bacteria breakingdownthe sugars in milk and releasinglactic acid (distinct tart/sour taste) b. Alcoholic fermentation- yeast and bacteria beingadded to tea and fruit (sugar), resultinginkombucha withethanol and carbondioxide bubbles
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Immune system {immunology) Immunity: is the ability of the body that defend against disease causing agents from the body such as bacteria, fungi, virus and e.t.c Immunology: is the study of the physiological defenses in which the body destroys or neutralizes foreign matter (both living and nonliving things). Immune defense system perform the following functions in below I. It protects against foreign microbes from the body. II. It Isolates or removes from the body non microbial foreign substances. III. It destroys cancer body cells by functional process called immune survaleince. Immunity can be divided into two types of defenses as fallowing in below. 1. Nonspecific defense (innate immunity or genetic defense). Nonspecific defense: it is the immunity or body’s defense mechanism that protects against any foreign or cancer cells without need recognizing their specific characteristics. Its mechanisms of protection are not unique for specific pathogens, so that it is called nonspecific defense. 2. Specific defense (acquired immunity). Specific immunity: it is the immunity that has specific recognition for unique or particular micro-organisms that is done by lymphocytes. For example Immunity created by vaccination Nonspecific of defense Nonspecific of defense consists of two of defenses of lines as fallowing in below  First line of defense: it is the first barrier against entry of foreign microorganisms to the human body. It include the following parts in below Walls and moats (surface defense); these are the first line of defense that consists of walls such as skin, interferon, nkc,, phagocytes, and moats such as mucus in tracts of the body. i. Walls (skin):- it is the outer most layer of the body and it is the first barrier against penetration of microbes, for example skin. The skin it has oil and sweat glands that give skin surface a PH of the 3 to 5 (acidic), Skin prevents excessive loss of water from the body by evaporation. ii. The Moats: - these are the mucous membranes in respiratory and digestive tracts Moats consist of digestive tract, respiratory tract and blood clotting. Prepared by Ahmed A/rahmaan Ahmmed Page 2 Digestive tract it has saliva which contain lysozyme (acidic) and other intestinal enzyme which kill microbes in the food. a. Respiratory tract The cells in the trachea secrete thick sticky mucus that traps microbes and dust in inhaled air. Also the cilia lining in this passage sweep the mucus containing germs towards glottis with help coughing, sneezing to expel germs out of the body b. Blood clotting: clotting prevents entry of microorganisms and seals the wounds c. Tear: tear secreted by lacrimal glands contain lysozyme enzyme that dilutes and digest microbes d. Saliva: saliva washes microbes from surface of the mouth and it contains amylase enzyme e. Urine: urine cleans the pathway of urethra in both males and females and it contains uric acid f. Sebum: oily substance secreted by sebaceous glands make clean on the surface of skin g. Hydrochloric acid:  Roaming and patrolling (second line of defense): it is second phase of the nonspecific immunity that is also called cellular counter attack defense It is the defense that consists of some white blood cells and chemical substances It responds to any microbial infection of invaders. ACTORS OF SECOND LINE DEFENSE Interferons, Phagocytes, natural killers, complement proteins, inflammations and fever are the common actors of second line defense. These cells are distributed through all the body to fight infections and they are also stored in lymphatic system. This defense works when the microorganisms breached through first line of defense. The cells that destroy antigens All the cells are originated from the bone marrow and there are two types of WBC defense. Granulocytes (phagocytes) A Granulocyte (lymphocytes) Neutrophils basophils eosinophils B cells T cells Nkc Origin and mode of action of phagocytes Phagocytes are nuetrophiphils they are produced and matured in bone marrow.  Neutrophils are the most numerous of leukocytes, they form 60% of wbc.  They are stored in bone marrow before maturation  Neutrophils increase in a number during infection times Prepared by Ahmed A/rahmaan Ahmmed Page 3  They travel through the body by squeezing the walls of capillaries towards the site of infection.  They ingest and kill bacteria by phagocytosis by releasing H2O2 and lysozyme enzymes.  They kill bacteria they form bus, so they are short lived cells. Monocytes  Monocytes are immature macrophages in bone marrow  Monocytes leave the bone marrow and get into blood stream where they grow in size and become mature now they are macrophages.  When they mature and settle in organs such as lungs, liver and lymph nodes they are called macrophages  They initiate immune response  They are long lived cells  Macrophage means big eaters. Phagocytosis: is the process in which neutrophils or macrophages engulf bacteria and destroy it STEPS OF Phagocytosis 1. Chemotaxis: is the process in which phagocytes attract and detect bacteria with helping of activating factor such as histamine. 2. Attachment: the phagocytes and becteria attach together, 3. Vacuole of neutrophils is formed 4. Endocytosis occurs endocytosis: is the process in which neutrophils or macrophages engulf bacterial cell, at lastly dead body cell, bacteria and dead neutrophils become bu
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