Extraction and Analysis of Total RNA from HeLa Cells

These flashcards cover key concepts and tasks related to the practical on RNA extraction and analysis from HeLa cells.

What microscopy technique is used to monitor HeLa cells?

Inverted Phase Contrast Microscopy.

What reagent is mentioned for isolating RNA from cells?

Tri-Reagent.

What is a critical step to avoid when working with RNA?

Avoid RNase contamination.

What is Task 1 in the practical regarding RNA?

Quantification of RNA concentration (µg/µl) by absorbance.

What does Task 2 focus on in the analysis of RNA samples?

Qualification of RNA sample on an Agarose gel.

Why might only ribosomal RNA be visible in extracted RNA?

Most abundant type of rna, mRNA is only 5%

What is an indication of high quality RNA after extraction?

The presence of distinct ribosomal RNA bands on a gel.