Biology practical

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proteus spp. possess ________ which they use for locomotion

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1

proteus spp. possess ________ which they use for locomotion

peritrichous flagella

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2

precipitates material onto the flagella, which makes them thicker and able to see with light microscopy

flagella stain

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3

noted for its tendency to swarm across an agar plate as it grows

proteus mirabilis

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4

Why is proteus mirablis troublesome to work with

it will grow over everything

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5

what is sometimes used to inhibit proteus mirabilis in order to allow the isolation of other species in a mixed culture

specialized media

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6

depends on oxygen for aerobic respiration

obligate aerobe

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7

what are the environmental factors that affect bacterial growth

oxygen and temperature

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8

respire aerobically, but are damaged by atmospheric oxygen concentrations

microaerophiles

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9

can respire aerobically, but use alternative strategies in anaerobic environment

falcultative anaerobes

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10

do not use oxygen but can survive and grow in its presence; not harmed by it

aerotolerant anaerobes

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11

cannot use oxygen and are harmed by its presence

strict anaerobes

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12

one way of accessing a bacterium's need for oxygen is to grow it in a tube of special medium, called

Thyoglycolate medium

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13

dissolved oxygen towards the top of the tube, but oxygen is absent in the deep part of the tube

Thyoglycolate medium

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14

obligate aerobes will grow

at the top

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15

microaerophiles will grow

slightly below the surface

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16

falcultative anaerobes will grow

throughout the media, but with heavier growth in the aerobic portion

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17

aerotolerant anaerobes will grow

throughout the media

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18

strict anaerobes will grow

towards the bottom of the tube where there is little to no oxygen

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19

will grow at 30° C, but may growth sparingly at 55° C

Bacillus subtillis and cereus

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20

will grow at 55°C, but will not grow at 30° C

Geobacillus stearothermophilus

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21

will grow at 30°C, but not at 55°C

Bacillus mycoides

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22

what colonies to Bacillus subtillis and cereus form

large, white ones

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23

on sheep blood agar, Bacillus cereus is

beta-hemolytic

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24

what do Bacillus mycoides look like when they growth

curly pattern, rhizoid

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25

produces red pigment prodigiosin when incubated at room temperature

Serratia marcescens

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26

non-water soluble pigment

prodigiosin

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27

prodigiosin at 37°C produces what color

clear

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28

prodigiosin in 95% ethanol produces what color

light red

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29

bright pink

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30

what are the pigment producers we encountered in lab

pseudomonads and Serratia marcescens

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31

a soil and water bacterium that is responsible for lung infections in cystic fibrosis patients,, skin infections in burn patients, hot tub rash, and swimmers ear

Pseudomonas aeruginosa

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32

what is the solubility for prodigiosin

non water soluble

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33

blue-green pigment whose production can be enhanced by Pseuomonas P agar

pyocyanin

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34

yellow-green flourescent pigments, whose production can be enhanced by Pseudomonas F agar

flourescein

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35

substances that can bind with iron and make it easier for organisms to absorb it

siderophores

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36

partial destruction of red blood cells

alpha hemolysis

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complete destruction of red blood cells

beta hemolysis

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non-destruction of red blood cells

gamma hemolysis

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39

1 stage micrometer =

.01 mm or 1 mm

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40

single flagellum

monotrichous

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41

has flagella at both ends

amphitrichous

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42

tuft of flagella at one or both ends

lophotrichous

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43

has flagella surrounding the cell

peritrichous

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44

extremely heat resistance structure produced only by a few bacterial genera

endospore

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45

transfering bacteria to fresh media

inoculation

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46

designed to allow for the isolation of single colonies of bacteria

4-quadrant streak

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47

the minimum distance between two objects which still allows them to be seen as separate

resolution

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48

Resolution is improved when the index of refraction of the medium between the slide and the objective is _______

increased

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49

the sharpness of the image being viewed

focus

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50

the same focal plane position is generally correct for all the objectives

parfocal

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51

used for motion

bacterial flagella

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52

Flagella can be up to ________ long, sometimes up to _________.

5-30 mm, 60 mm

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53

Designed to keep lab organisms from getting out and outside organisms from getting into the plate/slant etc.

aseptic techniques

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54

not a pigment produced by bacteria.

gram stain

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55

what are the 3 types of bacteria we worked with this year?

gram postive rods, gram positive cocci, gram negative rods

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56

attaches the cells firmly to the slide and keeps cell shape constant so that it does not become distorted during staining

heat fixation

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count dead cells and debris

density measurments

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58

counts bacteria that are able to reproduce and create a colony

viable count method

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59

what ciolor does Staphylococcus aureus belong to _____________ carotenoid pigment protects bacteria from host immune response, and plays a role in its virulence as pathogen.

Staphyloxanthin

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60

what color does Micrococcus luteus belong to ____________ carotenoid pigment, protects bacteria from stresses and host immune systems. Not linked to virulence, non water soluble

Sarcinaxanthin

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61

what colo does Micrococcus roseus belong to , non water soluble

Canthaxanthin

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62

what color does Chromobacterium violaceum belong to , antimicrobial properties and protection and non water soluble

Violacein

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63

water soluble dyes

will not penetrate spores

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64

what color belongs to Serratia marcescens and is produced at room temperature, not produced at 37C

Prodigiosin,

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65

what is prodigisn soluble in

methanol and chloroform

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Pseudomonas aeruginosa

pysocyanin

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67

Found in many parts of the body, skin

staphylococci

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68

found in the intestines

streptococci

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strep throat

Streptococcus pyogenes

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70

Media that favor the growth of some bacteria and disfavor the growth of others

selective media

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71

Media that favors the growth of all bacteria

differential media

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72

what type of media is SBA

differential

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73

MSA is __________ for halotolerant bacteria (staphylococci), and is also __________ due to mannitol fermentation.

selective, differential

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74

mannitol soy agar test

agar red will turn yellow

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75

the ability of an organism to grow at salt concentrations higher than those required for growth

halotolerant

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76

What type of media is MacConkey agar (MAC)?

differential and selective

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77

What type of media is Eosin Methylene Blue (EMB)

differential and selective

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78

What type of media is ]Triple Sugar Iron Agar (TSIA)

differential

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79

What are the subgroups of Lactose fermenters

Escherichia coli and Enterobacter

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80

what sugars make up TSIA slants

.1% glucose, 1% sucrose, 1% lactose

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81

Used to distinguish Enterobacter species and Escherichia coli

IMViC Tests

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82

What are the two E.coli strains

Strain I, recipient; Strain II, donor

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83

differentiates the beta hemolytic streptococci

bacitracin

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84

differentiaties the alpha hemolytic streptococci, by using pneumococci cells only

optochin

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85

differentiates gamma hemolytic staphylococci

novobiocin

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86

what does any zone of inhibition mean

positive (susceptible)

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87

what does no zone of inhibition mean

negative (resistant)

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88

how long does the zone of inhibition have to be for optochin

14 mm or greater

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how long does the zone of inhibition have to be for novobiocin

16 mm or greater

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90

determines the ability to cleave the amino acid tryptophan into indole and pyruvic

Indole test

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reveals what fermentation pathway the microbe uses to utilize glucose

Methyl Red Tet\st

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reveals if a microbe produces acetoin from glucose fermentation

The Voges Proskauer test

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reveals if a microbe uses sodium citrate as the only source of carbon and inorganic ammonium hydrogen phosphate as a source of nitrogen

The Simmons Ctitrate test

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94

abundance of bacteria that is found in the digestive tracts and waist of animals

coliforms

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95

streptomycin gene on chromosome; Cannot donate its resistance gene, not on a plasmid, no F factor.

Strain I, recipient

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96

ampicillin gene on plasmid, F factor

Strain II, donor

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97

Small circular double stranded DNA, separate from chromosome and can be transferred horizontally or vertically in a bacterial population

plasmid

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98

Bacteria possessing a plasmid and the F factor __________

(sex pilus)

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99

One bacterium transfers genetic material to another through direct contact

conjugation

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100

One bacterium transfers genetic material to another through direct contact _______

(pilus)

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