IE3 Biopharm: Enzymes (Foosh)

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23 Terms

1
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What is the effect of enzymes?

increase the rate of reaction by decreasing the activation energy

2
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What external factors that can affect the activity of enzymes?

optimum temperature and pH

3
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How does adenosine deaminase (ADA) prevent lymphocytoxity?

Adenosine deaminase (ADA) prevents lymphocytoxity by converting deoxyadenosine to deoxyinosine (deaminates/NH2 adenosine), → reduces deoxyadenosine levels that can inhibit T-cell activation and proliferation, inhibiting lymphotoxicity

<p>Adenosine deaminase (ADA) prevents lymphocytoxity by converting deoxyadenosine to deoxyinosine (deaminates/NH2 adenosine), → reduces deoxyadenosine levels that can inhibit T-cell activation and proliferation, inhibiting lymphotoxicity</p>
4
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How is SCID (severe combined immunodeficiency) treated?

enzyme replacement therapy w/ Elapegademase

5
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What is Elapegademase?

PEGylated recombinant adenosine deaminase (rADA) enzyme

6
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What is the structure of Elapegademase based on?

bovine amino acid sequence that is covalently conjugated, with a linker, to monomethoxypolyethylene glycol

7
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What is the disease that glucocerebrosidase deficiency leads to?

Gaucher’s disease

8
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What is Gaucher’s disease?

accumulation of certain fatty substances glucocerebrosides

9
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How is Gaucher’s disease/glucocerebrosidase deficiency treated?

  1. Imiglucerase (Cerezym)

  2. Velagluceraase alfa (Vpriv)

10
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Imiglucerase (Cerezym) has a shorter or longer half life than Velagluceraase alfa (Vpriv)?

Imiglucerase (Cerezym) has a shorter half life (3.6 - 10.4 min) than Velagluceraase alfa (Vpriv) (11-12 min)

11
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A deficiency in galsulfase leads to?

accumulation of chondroitin-4 sulfate and dermatan

12
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What is a deficiency in galsulfase treated with?

naglazyme (recombinant galsulfase glycoprotein)

(Gals? Na, glaze)

13
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What is the function of B-D-galactosidase (aka Lactase)?

breaks down lactose (two rings) into galactose and glucose (each one ring)

<p>breaks down lactose (two rings) into galactose and glucose (each one ring)</p>
14
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What is the oncolytic enzyme used for the treatment of acute lymphoblastic leukemia?

L-asparaginase (limits tumor growth)

<p>L-asparaginase (limits tumor growth)</p>
15
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What are the three different biologics of L-Asparginase?

  1. Asparaginase (Erwinia [Recombinant]) (Rylaze)

  2. Pegaspargase (Oncaspar)

  3. Calaspargase Pegol (Asparlas)

(Rile up On my AS(p))

16
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What is the biologic pegaspargase (Oncaspar) conjugated with?

monomethoxypolyethylene

(M-M-P-E)

17
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Calaspargase Pegol (Asparlas) has a structure of L-asparaginase and monomethoxypolyethylene glycol (mPEG) linked via…?

a succinimidyl carbonate linker (mPREG… suck-on-my-dicc)

18
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What are the two biologics that are uricases (catalyze urate oxidase)?

  1. Rasburicase (Elitek)

  2. Pegloticase (Krystexxa)

19
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What is pegloticase (Krystexxa) used for?

treatment of gout

20
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What is rasburicase (Elitek) used for?

treat elevated levels of uric acid arising from leukemia/chemo

21
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What is rhDNase I used for?

treatment of cystic fibrosis

22
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What biologic is a form of rhDNase I?

Dornase Alfa (Pulmozyme)

<p>Dornase Alfa (Pulmozyme)</p>
23
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What is the typical concentration of dornase alfa (pulmozyme)?

2500 U/2.5 mL

<p>2500 U/2.5 mL</p>