Microbiology Final Review

These flashcards cover key concepts from microbiology focusing on Gram staining, selective media, hemolysis types, and specific biochemical tests.

What is the primary stain used in the Gram staining process?

Crystal violet

What is the purpose of the mordant in the Gram stain procedure?

To enhance the binding of the primary stain to the bacterial cell wall.

What is the decolorizer used in the Gram stain method?

Acetone

What color does Gram-positive bacteria appear after the Gram staining process?

Purple

What type of media is MacConkey agar?

Selective media for Gram-negative bacteria.

What do halophiles refer to regarding their environment?

Salt-loving organisms.

What does the term 'hemolysis' refer to in a microbiology context?

The breakdown of red blood cells by bacteria.

What color indicates alpha hemolysis on blood agar?

Greenish coloration.

What color indicates beta hemolysis on blood agar?

Clear zone around the colonies.

What does gamma hemolysis indicate?

No hemolysis of red blood cells.

What is indicated by a positive catalase test?

Bubbles form when hydrogen peroxide is added.

What does a negative catalase test indicate?

No bubbles form when hydrogen peroxide is added.

What does a positive oxidase test indicate?

A color change to blue when oxidase reagent is applied.

What does a negative oxidase test indicate?

No color change occurs after adding oxidase reagent.