AFB/Mycobacterium

studied byStudied by 0 people
0.0(0)
get a hint
hint

____ stain = hot stain

1 / 106

encourage image

There's no tags or description

Looks like no one added any tags here yet for you.

107 Terms

1

____ stain = hot stain

Ziehl-Neilsen

New cards
2

____ stain = cold stain

Kinyoun

New cards
3

____ = fluorescent stain

auramine-rhodamine

New cards
4

T/F: any number of AFB seen on a smear is significant

T

New cards
5

____ media: 60% egg, malachite green

Lowenstein-Jensen

New cards
6

____ aids in dispersing colonies in liquid media

Tween 80

New cards
7

AFB: takes _-_ ____ for growth

3-6 weeks

New cards
8

T/F: can test urine for AFB

T

New cards
9

T/F: can refrigerate specimens for AFB testing overnight

T

New cards
10

Specimen decontamination: N-acetyl-L-cysteine (NALC) = ____ agent

mucolytic

New cards
11

Specimen decontamination: NaOH = ___

decontaminant

New cards
12

Stain and report AFB slides within ___ ___ of processing

24 hrs

New cards
13

Centrifuge decontaminated specimen for ___ min at ___ rpm before using sediment for making smears and inoculating media

20; 3000

New cards
14

Cauliflower colonies on LJ media => ___ ___

Mycobacterium tuberculosis

New cards
15

Mycobacterium avium complex: ____ colonies on LJ

non-pigmented

New cards
16

Rapid growers: M. _, M. _, and M. ____

fortuitum; chelonae; abscessus

New cards
17

Photochromogens: M. _, M. _, M. _, and M. _

kansasii; marinum; asiaticum; simiae

New cards
18

Scotochromogens: M. _, M. _, M. _, and M. _

scrofulaceum; gordonae; szulgai

New cards
19

Rough and buff colonies, serpentine cording on culture: M. ___ and M. ___

tuberculosis; bovis

New cards
20

Niacin accumulation => M. ___

tuberculosis

New cards
21

Nitrate reduction => M. _, M. _, and M. ___

tuberculosis; kansasii; fortuitum

New cards
22

Tween 80 hydrolysis < 5 days: M. ___ and M. ___

kansasii; marinum

New cards
23

Tween 80 hydrolysis > 5 days: M. ___ and M. ___

tuberculosis; bovis

New cards
24

Tween 80 hydrolysis 5-10 days: M. ___

gordonae

New cards
25

Tellurite: M. ___ and all ____

avium; rapid growers

New cards
26

Growth on MAC: M. ___ and M. ___

fortuitum; chelonae

New cards
27

M. chelonae: nitrate positive or negative?

negative

New cards
28

____chromogen: produces pigment in light

photo

New cards
29

____chromogen: produces pigment in dark and light

scoto

New cards
30

Rapid grower: growth in < ___ ___

7 days

New cards
31

M. ____: 32 degrees C optimum temp

ulcerans

New cards
32

M. ___: 30 degrees C optimum temp

marinum

New cards
33

Which is susceptible to TCH: M. tuberculosis or M. bovis?

bovis

New cards
34

Mycobacteria: gram-___ to gram-___ bacilli

positive; neutral

New cards
35

T/F: mycobacteria may appear beaded when stained

T

New cards
36

Read ZN and Kinyoun (carbolfuchsin) stains at ___X

100

New cards
37

ZN and Kinyoun (carbolfuchsin) stains: view > ___ fields before calling the slide negative.

300

New cards
38

Counter stain for ZN and Kinyoun (carbolfuchsin) stains = ___ ___

methylene blue

New cards
39

Fluorochrome stains: strong ___ light source with a ___ filter.

blue; FITC

New cards
40

Read Fluorochrome stains at ___ or ___X

25; 40

New cards
41

Some strains of M. ____ do not stain w/ fluorochrome dyes.

fortuitum

New cards
42

5% ___ ___: use for specimens known to be contaminated w/ Pseudomonas.

oxalic acid

New cards
43

Mycobactera: stimulated by ___ concentrations of fatty acids and inhibited by ___ concentrations

low; high

New cards
44

Mycobacteria media: carbon source = ___

glycerol

New cards
45

Malachite green function:

inhibition of growth of contaminants

New cards
46

Lowenstein-Jensen (LJ) is ___-based media.

egg

New cards
47

Which allows earlier detection of mycobacteria: clear agar-based media (e.g. 7H11) or egg media (LJ)?

agar

New cards
48

Protect 7H11 media from heat and light to prevent release of ____ (toxic to mycobacteria)

formaldehyde

New cards
49

_-_% CO2 is required for growth on 7H11 and stimulates growth on egg media (LJ)

5; 10

New cards
50

American Thoracic Society (ATS) media: egg or agar?

egg

New cards
51

Petragnani media: egg or agar?

egg

New cards
52

Gruft (LJ modification): ___ ___-based media.

selective egg

New cards
53

Middlebrook media: egg or agar?

agar

New cards
54

Mitchison's: ___ ___-based media

selective agar

New cards
55

Selective media: _, _, and ___

Gruft; Mitchison's; Mycobactosel

New cards
56

Mycobactosel: ___ ___-based media

selective egg

New cards
57

Curved strands of bacilli = ____

cording

New cards
58

Growth rate determined by which one: time of subculture time of detection from clinical sample?

subculture

New cards
59

Optimal growth temperature for M. xenopi = ___ degrees C

42

New cards
60

Photochromogens: produce ___ pigment when exposed to light.

carotene

New cards
61

Photochromogens: ___ ___ taking pictures of the ___ in ___

Asian; monkey; ocean; Kansas

New cards
62

M. tuberculosis, M. avium-intracellulare complex, M. bovis, M. haemophilum, M. ulcerans, M. chelonae, and M. fortuitum photoreactive class = ____

nonphotochromogens

New cards
63

M. szulgai, M. gordoae, M. scrofulceum photoreactive class = ____

scotochromogens

New cards
64

Photochromogens = Runyon group ___

1

New cards
65

Runyon Group 1 = ____

photochromogens

New cards
66

Scotochromogens = Runyon Group __

2

New cards
67

Runyon Group 2 = ____

scotochromogens

New cards
68

Nonchromogens = Runyon Group __

3

New cards
69

Runyon Group 3 = ____

nonchromogens

New cards
70

Rapid growers = Runyon Group __

4

New cards
71

Runyon Group 4 = ____

rapid growers

New cards
72

Rapid growers are usually pigmented or nonpigmented?

nonpigmented

New cards
73

Most common biochemical test for M. tuberculosis = ____

niacin

New cards
74

Niacin accumulation: positive = ____ color

yellow

New cards
75

Niacin accumulation: results are most consistent when performed on ___-based media.

egg

New cards
76

Negative niacin result => repeat in ___ ___ because young and/or few colonies may give false-negative.

several weeks

New cards
77

Nitrate reduction (to nitrite): positive = ___ color

red

New cards
78

Nitrate reduction test: no color change => add zinc => pink color => ____ result

negative

New cards
79

M. tuberculosis: heat-stable catalase ____

negative

New cards
80

Heat-stable catalase: positive after heating to ___ degrees C

68

New cards
81

Tween 80 hydrolysis: positive = ___ color

pink

New cards
82

Rapid growers (except M. chelonae) are iron uptake ___

positive

New cards
83

Which rapid grower is iron uptake negative? M. ____

chelonae

New cards
84

M. fortuitum. M. chelonae, and M. abscessus are ___-day arylsulfatase positive.

3

New cards
85

M. marinum, M. kansasii, and M. xenopi are ___-day arylsulfatase positive.

14

New cards
86

M. kansasii, M. marinum, and M. tuberculosis are pyrazinamidase ___

positive

New cards
87

M. bovis is pyrazinamidase ___

negative

New cards
88

T/F: can use pyrazinamidase to distinguish M. tuberculosis and M. bovis.

T

New cards
89

M. scrofulaceum = urease ___ M. gordonae = urease ___

positive; negative

New cards
90

TCH distinguishes M. ____ from M. ____

tuberculosis; bovis

New cards
91

Most ____ grow in the presence of 5% NaCl in egg-based media

rapid growers

New cards
92

Which has poorer growth on LJ, M. tuberculosis or M. bovis?

bovis

New cards
93

Orange crystals of beta carotene within colony centers => M. ____

kansasii

New cards
94

M. kansasii: type of disease caused: skin or pulmonary?

pulmonary

New cards
95

Which photochromogen is nitrate reduction positive? M. ___

kansasii

New cards
96

Which photochromogen is catalase negative? M. ___

marinum

New cards
97

Which photochromogen is Tween 80 hydrolysis negative? M. ___

simiae

New cards
98

Which photochromogen is niacin production positive? M. ____

simiae

New cards
99

M. scrofulaceum and M. gordonae are nitrate ___

negative

New cards
100

M. szulgai and M. flavescens are nitrate ___

positive

New cards

Explore top notes

note Note
studied byStudied by 19 people
Updated ... ago
5.0 Stars(1)
note Note
studied byStudied by 38 people
Updated ... ago
4.5 Stars(2)
note Note
studied byStudied by 8 people
Updated ... ago
5.0 Stars(1)
note Note
studied byStudied by 13 people
Updated ... ago
5.0 Stars(2)
note Note
studied byStudied by 10 people
Updated ... ago
5.0 Stars(1)
note Note
studied byStudied by 1 person
Updated ... ago
5.0 Stars(1)
note Note
studied byStudied by 59 people
Updated ... ago
4.7 Stars(3)
note Note
studied byStudied by 61 people
Updated ... ago
5.0 Stars(2)

Explore top flashcards

flashcards Flashcard30 terms
studied byStudied by 36 people
Updated ... ago
4.5 Stars(2)
flashcards Flashcard23 terms
studied byStudied by 48 people
Updated ... ago
5.0 Stars(1)
flashcards Flashcard59 terms
studied byStudied by 97 people
Updated ... ago
5.0 Stars(1)
flashcards Flashcard73 terms
studied byStudied by 19 people
Updated ... ago
5.0 Stars(2)
flashcards Flashcard20 terms
studied byStudied by 2 people
Updated ... ago
5.0 Stars(1)
flashcards Flashcard37 terms
studied byStudied by 11 people
Updated ... ago
5.0 Stars(1)
flashcards Flashcard26 terms
studied byStudied by 3 people
Updated ... ago
5.0 Stars(1)
flashcards Flashcard501 terms
studied byStudied by 7 people
Updated ... ago
5.0 Stars(1)