Binary Fission

What processes do Prokaryotes divide?

Binary Fission

What is the formula to calculate Binary Fission?

N x 2^n
N squared = time/mean division
N = future time

What happens during the Lagphase?

The bacteria is not growing and needs to adapt to new food

What happens during the Expotential phase?

• The DNA and plasmids replicate

• The cell gets bigger and move to opposite ends of the cell

• The cytoplasm begins to divide and new walls begins to form

• The cytoplasm divides and two daughter cells are produced.

What happens during stationary phase?

Bacteria stops growing, meaning it ran out of something

What varies depending on the cell?

The number of plasmids.

What conditions are good for binary fission?

• Warm environment

• lots of nutrients

• Oxygen

Culturing Microrganisms: Practical

1. Stirilise the surface with alcohol

2. Stirilise the inoculating loop by passing it through a flame’(bursen burner) to prevent contamination

3. Open the bacteria solution the least as possible to prevent other types of bacteria from leaving/entering.

4. Get the bacteria using the stirilised inoculating loop

5. Pass the solution through a flame and close it.

6. Open the petri dish the smallest as possible to prevnt bacteria on the air from coming in.

7. Transfer the bacteria to the agar gel by gently rubbing the incoulating loop in a zigzag pattern in the surface of the agar gel.

8. Close the lid and pass the inoculating loop through the flame again to prevent contamination.

9. Tape the petri dish on the sides to secure it but also to provide some oxygen for binary fission.

10. Incubate the petri dish in a temperature below 25*c (in schools).

11. Store the petri dih upside down to prevnt bacteria from dripping on the agar gel.

What temperature are petri dishes incubated in labs and why?

37*c because it helps the bacteria grow faster since it is the temperature of the human body.