Transcription vs Translation

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7 Terms

1
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direction

transcription: mRNA made 5’ → 3’, adding to 3’ end

translation: proteins are made amino group → carboxyl group, adding to COOH; ribosome is moving on template 5’ → 3’

2
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bond made

transcription: phosphodiester from 3’ C end of previous nucelotide to 5’ C of incoming nucleotide

translation: peptide

3
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enzyme

transcription: RNA polymerase

translation: ribosome

4
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nucleic acid pairing

transcription: DNA template to RNA, complementary base pairing

translation: RNA:RNA, nucleotide pairing, mRNA:tRNA

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where

transcription: nucleus

translation: cytoplasm

6
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how does it start

transcription: transcription factors (sigma bond, TBP and others) bind to promoter

translation: initiation factors (Shine-Delgarno sequence, 5’ cap, start codon) tell small ribosomal subunit and tRNA where to bind, then large subunit binds

7
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how does it stop

transcription: DNA

translation: stop codon (mRNA)