Flow Charts- (HPLC, GC-FID, GC-MS, & FAA)

Flashcards covering key concepts from the lecture notes on HPLC, GC-FID, and GC-MS techniques.

What are the four main steps in HPLC analysis?

Sample → Injection → Detection → Data Interpretation

What type of column is used in HPLC for separation?

C18 column (nonpolar)

What method is used for detection in HPLC?

UV-Vis DAD at λmax

How is concentration determined in HPLC?

Using area under the peak and calibration curve.

In GC-FID, what is the typical sample volume injected?

1–2 μL via split injector.

What type of detection method is used in GC-FID?

Detection by ion current in Hydrogen-Air flame.

What is the relationship between peak area and concentration in GC-FID?

Peak area is proportional to concentration.

What kind of solvent is typically used for sample preparation in GC-MS?

Volatile solvent (e.g., hexane).

What happens to the sample after it is injected into the GC in GC-MS?

It is ionized by Electron Ionization (EI) at 70 eV and fragmented.

What is the purpose of the Quadrupole mass filter in GC-MS?

To achieve m/z separation of ionized fragments.

Where can the extracted mass spectra be matched?

In the NIST library.

What key step follows atomization in the flame for metal analysis?

Emission of specific wavelengths for the respective metal.

How is concentration calculated in the method for aqueous metal samples?

Using standard addition or calibration.