Chemical Methods of Microbial Control

Chemotherapeutic drug

any chemical used in the treatment, relief or prophylaxis of a disease

prophylaxis

use of a drug to prevent potential infection in a person at risk

Antimicrobial chemotherapy

the use of chemotherapeutic drugs to control infection

Antimicrobials

any antimicrobial drug regardless of its origin

Antibiotic

a substance produced by microbes that can inhibit growth or destroy other microbes

semisynthetic drugs

compounds chemically modified in the laboratory after being isolated from natual sources

synthetic drugs

compounds synthesized entirely in the laboratory through chemical reactions

Minimum inhibatory concentration

lowest concentration of an antibiotic that prevents visible bacterial growth

Kirby-Bauer Method

uses antibiotic containing disk to test whether particular bacteria are susceptible to specific antibiotics

Kirby Bauer media

mueller-hinton agar

kirby bauer pH

7.2-7.4

Kirby Bauer age of culture for setup

2-6 hours

Incubation time Kirby Bauer

18-24 hours

Incubation temp

35-37

spreding tecnique

bacterial lawn

resistant

antibiotic has no effect

intermediate

antibiotic is only moderately effective (small ZOI)

sensitive

antibiotic is effective (large ZOI)

Broad Spectrum antibiotics

effective against a large range of bacteria

Narrow spectrum antibiotics

effective against a small group of bacteria

bactericidal

kills bacteria

Bacteriostatic

inhibit reproduction of bacteria allows immune system to eliminate them