megasheet - oxidative phosphorylation

electron transport chain (etc)

located in inner membrane space - creates [H+] gradient for atp synthase

electron transport chain (etc) - details

passes electrons from NADH/FADH oxidation to carriers - exergonic energy from reactions transports [H+] ions across

oxidative phosphorylation location

inner mitochondrial membrane - intermembrane space > matrix

intermembrane space characteristics (2)

high [H+] gradient, low ph (acidic)

mitochondrial matrix characteristics (2)

low [H+] gradient, high ph (basic)

transmembrane proteins in oxidative phosphorylation

1, 3, 4, atp synthase

transmembrane protein is also known as…

enzyme, protein pump

transmembrane proteins function

pump protons from matrix > inner membrane space with energy from electrons

oxidative phosphorylation - step 1

NADH - oxidized into NAD+ > electron donated to proton pump 1

oxidative phosphorylation - step 1.1

1 electron - carries over 1 proton [H+] from matrix > innermembrane space

oxidative phosphorylation - electron energy

energy being used from electron to transfer protons - electron's energy decreases

oxidative phosphorylation - step 2

same electron - transferred to proton pump 3 - carries over another [H+]

oxidative phosphorylation - step 3

same electron - transferred to final proton pump 4 - carries over a [H+]

oxidative phosphorylation - step 3.1

electron - transferred to O2 (final electron acceptor) - reacts with 4H+ to form H2O

oxidative phosphorylation - step 4

large [H+] in intermembrane space - carried to matrix through atp synthase against its concentration gradient

oxidative phosphorylation - step 5

mechanical energy from proton [H+] > phosphorylates adp + phosphate group > atp

oxidative phosphorylation - FADH

similar to NADH - FADH oxidized by etc - forms FAD2+ - electron donated to proton pump

oxidative phosphorylation - NAD+, FADH2+

nad and fad2+ - move back to be reduced (gain e-) in glycolysis, pyruvate oxidation, krebs cycle

1 NADH - electrons, atp result

1 nadh = 3 electrons, 3 atp

1 FADH2 - electrons, atp result

1 FADH2 = 2 electrons, 2 atp