Benedicts test for reducing sugars
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11 Terms
What is a reducing sugar?
All monosaccharides and some disaccharides are reducing sugars (can donate electrons or reduce another molecule)
What chemical is used?
Benedict’s reagant
What is the 1st step of the test?
Place the sample to be tested in a boiling tube, if not liquid grind it up
What is the 2nd step of the test?
Add an equal volume of Benedict’s
What is the 3rd step of the test?
Heat the mixture gently in a boiling water bath for 5 minutes
What reaction will occur?
Reducing sugars react with copper ions in benedict’s, addition of electrons to blue Cu2+ ions reducing them to brick red
What is a positive result?
A brick red precipitate forming
What does green colour indicate?
Low conc of reducing sugar
What does yellow/orange colour indicate?
Medium conc of reducing sugar
What does red colour indicate?
High conc of reducing sugar
What kind of test is it?
Qualitative
Note 
Flashcards (106)