Introduction to Spectrophotometry - EG
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10 Terms
Spectrophotometry
a scientific technique that measures the amount of light absorbed or transmitted by a sample
What does the spectrophotometer allow us to determine?
concentration of a substance
purity of a substance
composition of a substance
Light is a form of…
radiation / electromagnetic energy
Wavelengths
the different sizes energies of light / radiation comes in
the distance between peaks of a wave
Visible light
a form of electromagnetic radiation energy
appears in form of waves
How is the change in wavelength of visible light perceived?
perceived as different colors
Why does an object appear colored?
because it absorbs certain wavelengths of light and transmits/reflects others
you see the reflected colors of the object
How wil the absorption of an object appear?
it will appear in the form of a wavelength measured in nm (ex. chlorophylls)
Absolute maximum
the wavelength at which absorbance is the greatest
Stock solution
concentrated solution which needs to be diluted to less concentrated when needed
