Gene Expression, Protein Production, and Degradation: Transcription in Prokaryotes

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30 Terms

1
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How many subunits compose an RNA polymerase enzyme?
5
2
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What allows RNA polymerase to bind dsDNA?
Sigma
3
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Which component opens the closed complex into the open complex?
Sigma factor
4
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RNA nucleotides enter through a channel in which subunit?
Beta’
5
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What allows Rho to translocate along mRNA?
Conformation changes within its subunits and ATP hydrolysis.
6
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In prokaryotes, several different types of RNA polymerases perform transcription.
True
7
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What components of the RNA polymerase holoenzyme interact with dsDNA during promoter recognition?
Sigma factor and alpha
8
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Intrinsic termination is achieved by:
the mRNA transcript iself
9
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RNA polymerase contains two beta subunits.
False
10
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Which of the following is and RNA polymerase subunit?
Beta
11
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Each Rho subunit undergoes a continuous cycle of:
ATP-binding, ATP hydrolysis, release of ADP+Pi
12
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To terminate transcription, Rho translocates along the mRNA transcript:
towards RNA polymerase
13
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Rho can adopt:
a lock washer conformation
14
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The Rho monomers are arranged in:
a circle
15
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Rho-dependent termination is:
similar to intrinsic termination but instead involves a protein called Rho
16
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Which region(s) exist at the end of the mRNA transcript?
G-C rich region and U-rich region
17
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How long are the aborted transcripts?
About 10 bases
18
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What shape is created by the folding of the region near the end of the mRNA transcript?
Hairpin
19
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RNA polymerase contains two alpha subunits.
True
20
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Rho is a:
hexamer
21
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How many Rho subunits are in contact with mRNA upon initial binding?
6
22
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The same sigma factor is used for transcription in all genes.
False
23
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Which region folds onto iself?
G-C rich region
24
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Why is is the first transcript aborted?
Sigma factor is blocking the exit channel
25
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The Rho monomers are:
All the same
26
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Why is the U-rich region easily pulled off the DNA template?
It is weakly bound to the template
27
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Complimentary RNA bases are added started from what position?
\+1
28
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Rho contains:
a central channel, through which mRNA will pass
29
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The promoter regions consists of:
\-10, -13, UP element
30
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What is the difference between the closed complex and open complex?
The closed complex contains dsDNA that has not been separated, whereas in the open complex, dsDNA has been separated into two ssDNA strands.