Experiment 17: Paper Chromatography

Paper chromatography

Analytical method used to separate colored chemical or substances

Stationary and mobile phase

What are the two phases of Paper chromatography?

Mobile phase or carrier

Solvent moving through the column

Stationary phase or adsorbent

Substance that stays fixed inside the column

Eluent

Fluid entering the column

Eluate

Fluid exiting the column

Eluation

Process of washing out a compound through a column using a suitable solvent

Analyte

Individual components that is to be separated and analyzed

Capillary action

Phenomenon where the solution will begin to rise up the paper

Ascending paper chromatography

What type of paper chromatography is used in the experiment?

Stationary phase

Structure that holds the substance tested?

Filter paper

What is the stationary phase in the experiment?

Mobile phase

Solvent that flows through the stationary phase

Developing solution or solvent

What is the mobile phase in the experiment?

Retention factor

What is Rf?

Distance traveled by substance / distance traveled by solvent

Retention factor formula

4 butanol

1 acetic acid

5 distilled water

What is the component of the developing solution/solvent?

Aluminum foil

What is used to cover the beaker?

Covers the beaker to let the atmosphere be saturated with solvent vapor

Purpose of aluminum foil in the experiment

Lysine

Aspartic acid

Glycine

Amino acids used in the experiment

Ninhydrin

What was used to react with amino acid to form a colored compound?

Ninhydrin

Used as a drying agent

Whatmen filter paper

What kind of filter paper is used as paper chromatogram?

Oven

Set-up used to heat the filter paper