Exercise 5 - Isolation of Pure Culture of Organisms

Serial Dilution Pour Plate Method

Identify the method of isolating pure culture

Quebec Colony Counter

Identify equipment labeled B used in this test

Four Quadrant Streak Plate Method

Identify the method of isolating pure culture.

Nutrient Agar

What is the culture medium used in this test?

Melted Nutrient Agar

What is the culture medium used in this test?

Four Quadrant Streak Plate Method

Identify the method of isolating pure culture. (Answer correctly following the term in the laboratory briefing or handout material)

FALSE

The average diameter of petri dish is included in the computation of the colony forming units in this method. (TRUE or FALSE)

Quebec Colony Counter

What equipment is used in the counting of pure colonies in this method?

Serial Dilution Pour Plate Method

Identify the method of isolating pure culture. (Answer completely)

Fourth Quadrant

At which quadrant are pure colonies obtained?

Four-quadrant streaking

Identify technique used in this test

Qualitative Method

Four Quadrant Streak Plate Method

Quantitative Method

Serial Dilution Pour Plate Method

Interrupted Four-Quadrant Streaking

Contaminated specimen:

Continuous Four-Quadrant Streaking

Sterile Specimen

1 milliliter of inoculum and 9 milliliter of broth

How do you get 1:10 dilution

Ten-folds dilution

Series of dilution

Inversely proportional

Relationship of dilution to the number of colonies

Pressure-sensing device

Principle of Quebec Colony Counter

1:10, 1:100, 1:1000

Too Numerous to Count:

1:10,000, 1:100,000, 1:1,000,000

Can see single colonies:

Colony forming units per milliliter = colony count x reciprocal of dilution

Small number of colonies formula:

(1:10,000, 1:100,000, 1:1,000,000)

Colony forming units per milliliter = average number of colonies in 5 squares x 62.5 x reciprocal of dilution

Too numerous to count formula:

(1:10, 1:100, 1:1000)

62.5

Area of Petri dish

37 degree Celsius for 24 hours

Incubation period

Aseptic technique

Purpose of Alcohol Lamp

Quebec Colony Counter

What material is used in the quantitation of colonies?