Stem Cells

self renewal

mitotic division which creates the same type of cell as the mother stem cell

Why/how label S phase

Identify dividing cells, use Brad or Edu (thyme analogues which can be identified with antibodies)

iPSC

induced pluripotent stem cells

what type of stem cell is an embryonic stem cell

iPSC

Pluripotent

can produce cells of the embryo but not extra embryonic tissue

Multipotent

not pleura or totipotent but can generate several stem cell types in the tissue it is in

what is the difference between senescence and quiensence

senescence - triggered stable cell cycle arrest, can happen in G1 or G2, often triggered by stress

quiescence - G0, reversible

Transit amplifying cells

Undifferentiated cels following a stem cell division where quick increase in cell number is needed

G1

duplication of all; cellular components that aren’t chromosome

s

duplication of chromosome

G2

chromosome duplication checks

immunocytochemistry

use of fluorescent antibodies on cell

immunohistochemistry

use of fluorescent antibodies on tissues

nanog 4

Tf for self renewal of stem cells

ssea1

plurioptency markerUTF

UTF1

spermatoginal stem cells

Oct4

plurpotency marker

CiPS

Chemically induced pluripotent stem cells

give a loading gene in RT-PCR

GAPDHPSLM

OSKM iPS

stem cells generated with OSKM factors (Oct4, Sox2, KL4, c-myc)

what is the difference between OSKM iPSCs and CiPS

OSKM- genetic modification with viral vectors, takes longer, oncogenes used.