lab quiz 2 practice test

Describe the colony morphology of S. marcescens

round shape, red color

Describe the colony morphology of E. coli

rough, irregular shape, pale white color

Describe the colony morphology of S. aureus

round shape, clear/white color, slightly raised

Describe the colony morphology of M. Luteus

round shape, yellow color

describe the contents of an example sterile tube

A sterile tube typically contains a liquid medium such as nutrient broth or agar and is free from any contaminants.

describe the contents of an example Pure tube

A pure tube contains a single type of microorganism in a sterile medium, ensuring no contamination from other species.

describe the contents of an example mixed tube

A mixed tube contains two or more different types of microorganisms in a sterile medium, allowing for the growth of multiple species within the same environment.

describe the contents of an example contaminated tube

A contaminated tube contains one or more unwanted microorganisms, likely from external sources, compromising the sterility and integrity of the culture.

what is the charge of the bacteria in simple staining

negative

what is the charge of the bacteria for negative staining?

negative

t/f cells are not stained in negative staining

true

what are two disadvantages for heat fixation?

the bacteria can die off and disorts the bacteria

what are the 10 steps in correct sequence for simple staining?

1. add drop of water to slide

2. add bacterial culture

3. heat fixation

4. cool down slide

5. stain slide

6. wash with distilled water

7. blot with bibulous paper

8. air dry smear

9. ensure its dry

10. view under microscope

What are the 10 steps for a gram stain?

1. Prepare a heat-fixed smear 2. Stain with crystal violet 3. Rinse with water 4. Apply iodine mordant 5. Rinse with water 6. Decolorize with alcohol 7. Rinse with water 8. Counterstain with safranin 9. Rinse with water 10. Blot dry and view under microscope

what are the 9 steps for acid fast stain?

1. Prepare a heat-fixed smear 2. Stain with carbol fuchsin 3. Steam for 5 minutes 4. Rinse with water 5. Decolorize with acid-alcohol 6. Rinse with water 7. Counterstain with methylene blue 8. Rinse with water 9. Blot dry and view under microscope